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Literature summary extracted from

  • Bryant, F.O.; Wiegel, J.; Ljungdahl, L.G.
    Purification and properties of primary and secondary alcohol dehydrogenases from Thermoanaerobacter ethanolicus (1988), Appl. Environ. Microbiol., 2, 460-465.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.2 synthesis
-
Saccharomyces cerevisiae
1.1.1.2 synthesis
-
Acinetobacter sp.
1.1.1.2 synthesis
-
Zymomonas mobilis
1.1.1.2 synthesis potential use for industrial production of ethanol by fermentation, thermophilic fermentations offer the potential to separate ethanol from continous cultures at process temperature and reduced pressure during growth Thermoanaerobacter ethanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.022
-
NADP+ secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 0.15
-
NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 0.35
-
NADP+ secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 0.36
-
NADPH secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 0.83
-
NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 1.5
-
NADPH primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 16.4
-
propan-2-ol secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 16.4
-
ethanol primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 44.8
-
acetaldehyde secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 47.1
-
n-butanol primary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 49
-
ethanol secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus
1.1.1.2 210
-
acetaldehyde primary alcohol dehydrogenase Thermoanaerobacter ethanolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 42000
-
4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
1.1.1.2 44500
-
4 * 44500, primary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
1.1.1.2 172000
-
secondary alcohol dehydrogenase, gel filtration Thermoanaerobacter ethanolicus
1.1.1.2 184000
-
primary alcohol dehydrogenase, gel filtration Thermoanaerobacter ethanolicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Acinetobacter sp.
-
-
-
1.1.1.2 Clostridium beijerinckii
-
-
-
1.1.1.2 Escherichia coli
-
-
-
1.1.1.2 Geobacillus stearothermophilus
-
-
-
1.1.1.2 Saccharomyces cerevisiae
-
yeast
-
1.1.1.2 Thermoanaerobacter brockii
-
-
-
1.1.1.2 Thermoanaerobacter ethanolicus
-
strain ATCC 31550
-
1.1.1.2 Thermoanaerobacter thermohydrosulfuricus
-
-
-
1.1.1.2 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2 1° Adh and 2°Adh Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1,2-propanediol + NADP+
-
Thermoanaerobacter ethanolicus ? + NADPH
-
?
1.1.1.2 1,3-butanediol + NADP+
-
Thermoanaerobacter ethanolicus ? + NADPH
-
?
1.1.1.2 1,3-propanediol + NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus ? + NADPH
-
?
1.1.1.2 1-pentanol + NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus n-pentanal + NADPH
-
r
1.1.1.2 2,3-pentanedione + NADPH + H+ secondary alcohol dehydrogenase Thermoanaerobacter ethanolicus ? + NADP+
-
?
1.1.1.2 2-butanol + NADP+
-
Thermoanaerobacter ethanolicus butan-2-one + NADPH
-
r
1.1.1.2 2-butanol + NADP+
-
Thermoanaerobacter brockii butan-2-one + NADPH
-
r
1.1.1.2 2-heptanol + NADP+
-
Thermoanaerobacter ethanolicus heptan-2-one + NADPH
-
r
1.1.1.2 2-methyl-1-propanol + NADP+
-
Thermoanaerobacter ethanolicus 2-methylpropanal + NADPH
-
?
1.1.1.2 2-pentanol + NADP+
-
Thermoanaerobacter ethanolicus pentan-2-one + NADPH
-
r
1.1.1.2 3-hexanol + NADP+
-
Thermoanaerobacter ethanolicus hexan-3-one + NADPH
-
r
1.1.1.2 acetaldehyde + NADPH + H+
-
Acinetobacter sp. ethanol + NADP+
-
?
1.1.1.2 acetaldehyde + NADPH + H+
-
Thermoanaerobacter ethanolicus ethanol + NADP+
-
?
1.1.1.2 acetone + NADPH
-
Thermoanaerobacter ethanolicus 2-propanol + NADP+
-
?
1.1.1.2 ethanol + NADP+
-
Escherichia coli acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Geobacillus stearothermophilus acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Acinetobacter sp. acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Zymomonas mobilis acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Thermoanaerobacter thermohydrosulfuricus acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Clostridium beijerinckii acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Thermoanaerobacter ethanolicus acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Thermoanaerobacter brockii acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethylene glycol + NADP+
-
Thermoanaerobacter ethanolicus ? + NADPH
-
?
1.1.1.2 n-butanol + NADP+
-
Zymomonas mobilis butyraldehyde + NADPH
-
r
1.1.1.2 n-butanol + NADP+
-
Thermoanaerobacter ethanolicus butyraldehyde + NADPH
-
r
1.1.1.2 n-heptanol + NADP+
-
Saccharomyces cerevisiae n-heptanal + NADPH
-
r
1.1.1.2 n-heptanol + NADP+ primary alcohol dehydrogenase Thermoanaerobacter ethanolicus n-heptanal + NADPH
-
r
1.1.1.2 n-propanol + NADP+
-
Thermoanaerobacter ethanolicus propionaldehyde + NADPH
-
r
1.1.1.2 propan-2-ol + NADP+
-
Thermoanaerobacter ethanolicus propanone + NADPH
-
r
1.1.1.2 propionaldehyde + NADPH
-
Thermoanaerobacter ethanolicus propanol + NADP+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 tetramer
-
Acinetobacter sp.
1.1.1.2 tetramer
-
Thermoanaerobacter brockii
1.1.1.2 tetramer 4 * 44500, primary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus
1.1.1.2 tetramer 4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE Thermoanaerobacter ethanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 80
-
secondary alcohol dehydrogenase with 2-propanol as substrate Thermoanaerobacter ethanolicus
1.1.1.2 85
-
primary enzyme, 1-butanol as substrate Thermoanaerobacter ethanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 70
-
-
Acinetobacter sp.
1.1.1.2 70
-
no loss of activity during incubation for 2 h Thermoanaerobacter ethanolicus
1.1.1.2 80
-
secondary alcohol dehydrogenase with 2-propanol as substrate Thermoanaerobacter ethanolicus
1.1.1.2 85
-
primary enzyme, 1-butanol as substrate Thermoanaerobacter ethanolicus