EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.2 | synthesis | - |
Saccharomyces cerevisiae |
1.1.1.2 | synthesis | - |
Acinetobacter sp. |
1.1.1.2 | synthesis | - |
Zymomonas mobilis |
1.1.1.2 | synthesis | potential use for industrial production of ethanol by fermentation, thermophilic fermentations offer the potential to separate ethanol from continous cultures at process temperature and reduced pressure during growth | Thermoanaerobacter ethanolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.022 | - |
NADP+ | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.15 | - |
NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.35 | - |
NADP+ | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.36 | - |
NADPH | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 0.83 | - |
NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 1.5 | - |
NADPH | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 16.4 | - |
propan-2-ol | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 16.4 | - |
ethanol | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 44.8 | - |
acetaldehyde | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 47.1 | - |
n-butanol | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 49 | - |
ethanol | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | |
1.1.1.2 | 210 | - |
acetaldehyde | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 42000 | - |
4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.1.1.2 | 44500 | - |
4 * 44500, primary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.1.1.2 | 172000 | - |
secondary alcohol dehydrogenase, gel filtration | Thermoanaerobacter ethanolicus |
1.1.1.2 | 184000 | - |
primary alcohol dehydrogenase, gel filtration | Thermoanaerobacter ethanolicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Acinetobacter sp. | - |
- |
- |
1.1.1.2 | Clostridium beijerinckii | - |
- |
- |
1.1.1.2 | Escherichia coli | - |
- |
- |
1.1.1.2 | Geobacillus stearothermophilus | - |
- |
- |
1.1.1.2 | Saccharomyces cerevisiae | - |
yeast | - |
1.1.1.2 | Thermoanaerobacter brockii | - |
- |
- |
1.1.1.2 | Thermoanaerobacter ethanolicus | - |
strain ATCC 31550 | - |
1.1.1.2 | Thermoanaerobacter thermohydrosulfuricus | - |
- |
- |
1.1.1.2 | Zymomonas mobilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | 1° Adh and 2°Adh | Thermoanaerobacter ethanolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 1,2-propanediol + NADP+ | - |
Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
1.1.1.2 | 1,3-butanediol + NADP+ | - |
Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
1.1.1.2 | 1,3-propanediol + NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
1.1.1.2 | 1-pentanol + NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | n-pentanal + NADPH | - |
r | |
1.1.1.2 | 2,3-pentanedione + NADPH + H+ | secondary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | ? + NADP+ | - |
? | |
1.1.1.2 | 2-butanol + NADP+ | - |
Thermoanaerobacter ethanolicus | butan-2-one + NADPH | - |
r | |
1.1.1.2 | 2-butanol + NADP+ | - |
Thermoanaerobacter brockii | butan-2-one + NADPH | - |
r | |
1.1.1.2 | 2-heptanol + NADP+ | - |
Thermoanaerobacter ethanolicus | heptan-2-one + NADPH | - |
r | |
1.1.1.2 | 2-methyl-1-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | 2-methylpropanal + NADPH | - |
? | |
1.1.1.2 | 2-pentanol + NADP+ | - |
Thermoanaerobacter ethanolicus | pentan-2-one + NADPH | - |
r | |
1.1.1.2 | 3-hexanol + NADP+ | - |
Thermoanaerobacter ethanolicus | hexan-3-one + NADPH | - |
r | |
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Acinetobacter sp. | ethanol + NADP+ | - |
? | |
1.1.1.2 | acetaldehyde + NADPH + H+ | - |
Thermoanaerobacter ethanolicus | ethanol + NADP+ | - |
? | |
1.1.1.2 | acetone + NADPH | - |
Thermoanaerobacter ethanolicus | 2-propanol + NADP+ | - |
? | |
1.1.1.2 | ethanol + NADP+ | - |
Escherichia coli | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Geobacillus stearothermophilus | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Acinetobacter sp. | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Zymomonas mobilis | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Thermoanaerobacter thermohydrosulfuricus | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Clostridium beijerinckii | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Thermoanaerobacter ethanolicus | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | - |
Thermoanaerobacter brockii | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | ethylene glycol + NADP+ | - |
Thermoanaerobacter ethanolicus | ? + NADPH | - |
? | |
1.1.1.2 | n-butanol + NADP+ | - |
Zymomonas mobilis | butyraldehyde + NADPH | - |
r | |
1.1.1.2 | n-butanol + NADP+ | - |
Thermoanaerobacter ethanolicus | butyraldehyde + NADPH | - |
r | |
1.1.1.2 | n-heptanol + NADP+ | - |
Saccharomyces cerevisiae | n-heptanal + NADPH | - |
r | |
1.1.1.2 | n-heptanol + NADP+ | primary alcohol dehydrogenase | Thermoanaerobacter ethanolicus | n-heptanal + NADPH | - |
r | |
1.1.1.2 | n-propanol + NADP+ | - |
Thermoanaerobacter ethanolicus | propionaldehyde + NADPH | - |
r | |
1.1.1.2 | propan-2-ol + NADP+ | - |
Thermoanaerobacter ethanolicus | propanone + NADPH | - |
r | |
1.1.1.2 | propionaldehyde + NADPH | - |
Thermoanaerobacter ethanolicus | propanol + NADP+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | tetramer | - |
Acinetobacter sp. |
1.1.1.2 | tetramer | - |
Thermoanaerobacter brockii |
1.1.1.2 | tetramer | 4 * 44500, primary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
1.1.1.2 | tetramer | 4 * 42000, 2° Adh, secondary alcohol dehydrogenase, SDS-PAGE | Thermoanaerobacter ethanolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 80 | - |
secondary alcohol dehydrogenase with 2-propanol as substrate | Thermoanaerobacter ethanolicus |
1.1.1.2 | 85 | - |
primary enzyme, 1-butanol as substrate | Thermoanaerobacter ethanolicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 70 | - |
- |
Acinetobacter sp. |
1.1.1.2 | 70 | - |
no loss of activity during incubation for 2 h | Thermoanaerobacter ethanolicus |
1.1.1.2 | 80 | - |
secondary alcohol dehydrogenase with 2-propanol as substrate | Thermoanaerobacter ethanolicus |
1.1.1.2 | 85 | - |
primary enzyme, 1-butanol as substrate | Thermoanaerobacter ethanolicus |