Any feedback?
Literature summary extracted from
- L-Glycol dehydrogenase from hen muscle (1982), Methods Enzymol., 89, 523-526.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.185 | easily inactivated in high molarity buffers | Gallus gallus |
| 1.1.1.185 | very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.185 | acetone | pI 7.2 form | Gallus gallus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.185 | Gallus gallus | - |
hen | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.185 | 3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8 | Gallus gallus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.185 | an L-glycol + NAD(P)+ = a 2-hydroxycarbonyl compound + NAD(P)H + H+ | glyoxal reduction by enzyme form pI 7.2 follows ordered bi-bi mechanism in which the coenzyme is the first substrate to bind to the enzyme | Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.185 | muscle | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.185 | 0.4 | - |
- |
Gallus gallus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.185 | -18°C, enzyme at any stage of purification | Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.185 | R1-CO-CHOH-R2 + NAD(P)H | - |
Gallus gallus | R1-CHOH-CHOH-R2 + NAD(P)+ | - |
r | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.185 | 25 | - |
assay at | Gallus gallus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.185 | 5 | 6.6 | - |
Gallus gallus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.185 | 7 | - |
very stable in both water and low molarity buffers of pH 7 at 0-4°C | Gallus gallus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.185 | NAD+ | - |
Gallus gallus | |
| 1.1.1.185 | NADH | - |
Gallus gallus | |
| 1.1.1.185 | NADPH | - |
Gallus gallus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
