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Literature summary extracted from

  • Potty, V.H.; Bruemmer, J.H.
    Oxidation of geraniol by enzyme system from orange (1970), Phytochemistry, 9, 1003-1007.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.183 2-mercaptoethanol protects enzyme from inhibition Citrus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.183 (+)-limonene 100% inhibition at 0.488 mM Citrus sp.
1.1.1.183 8-hydroxyquinoline 50% decrease at 0.67 mM Citrus sp.
1.1.1.183 alpha,alpha'-dipyridyl 50% inhibition at 25 mM Citrus sp.
1.1.1.183 alpha-terpineol about 55% inhibition at 0.56 mM Citrus sp.
1.1.1.183 EDTA 50% inhibition at 67.7 mM Citrus sp.
1.1.1.183 iodoacetamide
-
 Citrus sp.
1.1.1.183 linalool about 55% inhibition at 1.12 mM Citrus sp.
1.1.1.183 o-phenanthroline 50% inhibition at 16.7 mM Citrus sp.
1.1.1.183 oxidized glutathione reaction completely stopped at 3.333 mM Citrus sp.
1.1.1.183 Sodium azide 50% inhibition at 83 mM Citrus sp.
1.1.1.183 Zn2+ 50% inhibition 1.67 mM Citrus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.183 0.0465
-
 geraniol pH 9.0 Citrus sp.
1.1.1.183 0.0833
-
 geraniol pH 7.0 Citrus sp.
1.1.1.183 0.454
-
 NADP+ pH 9.0 Citrus sp.
1.1.1.183 0.83
-
 NADP+ pH 7.0 Citrus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.183 Zn2+ suggested to be part of the enzyme Citrus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.183 92000
-
 estimation, gel filtration Citrus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.183 geraniol + NADP+ Citrus sp. key intermediate in terpenoid biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.183 Citrus sp.
-
 orange
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.183 partially, ammonium sulfate fractionation, gel filtration, anion exchange chromatography Citrus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.183 fruit
-
 Citrus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.183 additional information
-
 nerol shows 60% of the rate with geraniol, farnesol about 11% and citronellol 8% Citrus sp.
1.1.1.183 0.203
-
-
 Citrus sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.183 -19°C, dialyzed, rapid loss of activity Citrus sp.
1.1.1.183 -96°C, undialyzed ammonium sulfate fraction, 6 months, quite active Citrus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.183 citronellol + NADP+
-
 Citrus sp. citronellal + NADPH
-
 ?
1.1.1.183 farnesol + NADP+
-
 Citrus sp. farnesal + NADPH
-
 ?
1.1.1.183 geraniol + NADP+
-
 Citrus sp. geranial + NADPH
-
 r
1.1.1.183 geraniol + NADP+ key intermediate in terpenoid biosynthesis Citrus sp. ?
-
 ?
1.1.1.183 additional information nerol, farnesol and citronellol are oxidized at slower rates Citrus sp. ?
-
 ?
1.1.1.183 nerol + NADP+
-
 Citrus sp. neral + NADPH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.183 6.5
-
 geranial reduction Citrus sp.
1.1.1.183 9
-
 geraniol oxidation Citrus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.183 5 10 below 5 complete and irreversible inactivation after 5 min Citrus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.183 5 10 below pH 5 complete and irreversible inactivation after 5 min Citrus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.183 additional information NAD+ is ineffective Citrus sp.
1.1.1.183 NADP+
-
 Citrus sp.
1.1.1.183 NADPH
-
 Citrus sp.