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Literature summary extracted from

  • Conrad R.S.; Massey L.K.; Sokatch J.R.
    D- and L-leucine metabolism and regulation of their pathways in Pseudomonas putida (1974), J. Bacteriol., 4, 103-111.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.178 0.037
-
 2-methyl-3-hydroxybutyryl-CoA
-
 Pseudomonas putida
1.1.1.178 0.5
-
 3-hydroxybutyryl-CoA
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.178 130000
-
 gel filtration, sucrose density gradient centrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.178 2-methyl-3-hydroxybutyryl-CoA + NAD+ Pseudomonas putida isoleucine metabolism 2-methylacetoacetyl-CoA + NADH
-
 ?
1.1.1.178 2-methyl-3-hydroxybutyryl-CoA + NAD+ Pseudomonas putida PpG2 isoleucine metabolism 2-methylacetoacetyl-CoA + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.178 Pseudomonas putida
-
 PpG2
-
1.1.1.178 Pseudomonas putida PpG2
-
 PpG2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.178
-
 Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.178 (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH + H+ inducible by tiglate Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.178 16.2
-
 2-methyl-3-hydroxybutyryl-CoA Pseudomonas putida
1.1.1.178 21.7
-
 3-hydroxybutyryl-CoA Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.178 2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
 Pseudomonas putida 3-methyl-2-oxopentanoyl-CoA + NADH
-
 ?
1.1.1.178 2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
 Pseudomonas putida PpG2 3-methyl-2-oxopentanoyl-CoA + NADH
-
 ?
1.1.1.178 2-methyl-3-hydroxybutyryl-CoA + NAD+
-
 Pseudomonas putida 2-methylacetoacetyl-CoA + NADH
-
 ?
1.1.1.178 2-methyl-3-hydroxybutyryl-CoA + NAD+ isoleucine metabolism Pseudomonas putida 2-methylacetoacetyl-CoA + NADH
-
 ?
1.1.1.178 2-methyl-3-hydroxybutyryl-CoA + NAD+
-
 Pseudomonas putida PpG2 2-methylacetoacetyl-CoA + NADH
-
 ?
1.1.1.178 2-methyl-3-hydroxybutyryl-CoA + NAD+ isoleucine metabolism Pseudomonas putida PpG2 2-methylacetoacetyl-CoA + NADH
-
 ?
1.1.1.178 3-hydroxybutyryl-CoA + NAD+
-
 Pseudomonas putida acetoacetyl-CoA + NADH
-
 ?
1.1.1.178 3-hydroxybutyryl-CoA + NAD+
-
 Pseudomonas putida PpG2 acetoacetyl-CoA + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.178 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.178 9.5
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.178 NAD+ cannot be replaced by NADP+ Pseudomonas putida