EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.168 | 2-dehydropantoyl lactone + NADPH | Saccharomyces cerevisiae | - |
(R)-pantoyl lactone + NADP+ | - |
? | |
1.1.1.214 | additional information | Escherichia coli | the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.168 | Saccharomyces cerevisiae | - |
- |
- |
1.1.1.169 | Escherichia coli | - |
ATCC 9637 | - |
1.1.1.169 | Saccharomyces cerevisiae | - |
- |
- |
1.1.1.214 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.169 | gel chromatography | Escherichia coli |
1.1.1.169 | gel chromatography | Saccharomyces cerevisiae |
1.1.1.214 | form A and form B of the enzyme obtained separated by rupture, centrifugation and column chromatography on DEAE-cellulose and hydroxylapatite | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.168 | 2-dehydropantoyl lactone + NADPH | - |
Saccharomyces cerevisiae | (R)-pantoyl lactone + NADP+ | - |
? | |
1.1.1.168 | additional information | keto-omega-methylpantoyl lactone is the only analog of ketopantoyl lactone tested that is a substrate | Saccharomyces cerevisiae | additional information | - |
? | |
1.1.1.168 | additional information | not: several other 2-keto acids | Saccharomyces cerevisiae | additional information | - |
? | |
1.1.1.168 | additional information | not: ketopantoic acid | Saccharomyces cerevisiae | additional information | - |
? | |
1.1.1.169 | ketopantoic acid + NADPH | B-specific | Escherichia coli | pantoic acid + NADP+ | - |
? | |
1.1.1.169 | ketopantoic acid + NADPH | B-specific | Saccharomyces cerevisiae | pantoic acid + NADP+ | - |
? | |
1.1.1.214 | keto-omega-methylpantoyl lactone + NADPH | the only compound other than ketopantoyl lactone which is a substrate, 73% relative activity to ketopantoyl lactone with form A enzyme, 56% relative activity to ketopantoyl lactone with form B enzyme | Escherichia coli | methylpantoyl lactone + NADP+ | - |
? | |
1.1.1.214 | ketopantoyl lactone + NADPH | - |
Escherichia coli | pantoyl lactone + NADP+ | - |
? | |
1.1.1.214 | additional information | both forms of the enzyme are B-specific, the enzyme exhibits opposite stereospecificity from that observed with the Saccharomyces cerevisiae enzyme, which is A-specific, less than 5% relative activity to ketopantoyl lactone with the following 2-keto-gamma-lactones: 2-keto-4-hydroxy-3-methylbutyric acid-gamma-lactone, 2-keto-4-hydroxybutyric acid-gamma-lactone | Escherichia coli | ? | - |
? | |
1.1.1.214 | additional information | the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown | Escherichia coli | ? | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | NADPH | B-specific hydrogen transfer | Escherichia coli | |
1.1.1.169 | NADPH | B-specific hydrogen transfer | Saccharomyces cerevisiae | |
1.1.1.214 | additional information | no activity observed with NADH | Escherichia coli | |
1.1.1.214 | NADPH | - |
Escherichia coli |