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Literature summary extracted from

  • King, H.L.; Wilken, D.R.
    Separation and preliminary studies on 2-ketopantoyl lactone and 2-ketopantoic acid reductases of yeast (1972), J. Biol. Chem., 247, 4096-4098.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.168 2-dehydropantoyl lactone + NADPH Saccharomyces cerevisiae
-
 (R)-pantoyl lactone + NADP+
-
 ir
1.1.1.168 2-dehydropantoyl lactone + NADPH Saccharomyces cerevisiae NRRL Y-2034
-
 (R)-pantoyl lactone + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.168 Saccharomyces cerevisiae
-
-
-
1.1.1.169 Saccharomyces cerevisiae
-
 NRRL Y-2034
-
1.1.1.169 Saccharomyces cerevisiae NRRL Y-2034
-
 NRRL Y-2034
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.169 gel chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.169 additional information
-
 activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde Saccharomyces cerevisiae
1.1.1.169 0.38
-
 at pH 6.5 using NADPH and ketopantoic acid Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.168 2-dehydropantoyl lactone + NADPH
-
 Saccharomyces cerevisiae (R)-pantoyl lactone + NADP+
-
 ir
1.1.1.168 2-dehydropantoyl lactone + NADPH
-
 Saccharomyces cerevisiae NRRL Y-2034 (R)-pantoyl lactone + NADP+
-
 ir
1.1.1.168 additional information not: ketopantoic acid Saccharomyces cerevisiae additional information
-
 ?
1.1.1.168 additional information not: ketopantoic acid Saccharomyces cerevisiae NRRL Y-2034 additional information
-
 ?
1.1.1.169 ketopantoic acid + NADPH
-
 Saccharomyces cerevisiae pantoic acid + NADP+
-
 ?
1.1.1.169 ketopantoic acid + NADPH
-
 Saccharomyces cerevisiae NRRL Y-2034 pantoic acid + NADP+
-
 ?
1.1.1.169 additional information activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde Saccharomyces cerevisiae ?
-
 ?
1.1.1.169 additional information activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde Saccharomyces cerevisiae NRRL Y-2034 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.168 7
-
-
 Saccharomyces cerevisiae
1.1.1.169 5
-
 reduction of ketopantoic acid with NADPH Saccharomyces cerevisiae
1.1.1.169 5.5
-
 reduction of ketopantoic acid with NADH Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.168 5.2 8 at pH 5.2 and 8.0 about 50% of activity maximum Saccharomyces cerevisiae
1.1.1.169 4.3 6.5 pH 4.3: about 10% of activity maximum, pH 6.5: about 40% of activity maximum Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 NADH less active than NADPH at 0.1 mM Saccharomyces cerevisiae
1.1.1.169 NADPH
-
 Saccharomyces cerevisiae