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Literature summary extracted from

  • Shimizu, S.; Kataoka, M.; Ching-Ming Chung, M.; Yamada, H.
    Ketopantoic acid reductase of Pseudomonas maltophilia 845. Purification, characterization, and role in pantothenate biosynthesis (1988), J. Biol. Chem., 263, 12077-12084.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.169 fine needle, at about 40% ammonium sulfate saturation Stenotrophomonas maltophilia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.169 Barbital 9.0% at 1 mM Stenotrophomonas maltophilia
1.1.1.169 D-pantoic acid noncompetitive with ketopantoic acid at saturation level of NADPH, noncompetitive with NADPH at unsaturation level of ketopantoic acid Stenotrophomonas maltophilia
1.1.1.169 Diphenylhydantoin 22.6% at 1 mM Stenotrophomonas maltophilia
1.1.1.169 iodoacetate sulfhydryl reagents, slightly inhibitory Stenotrophomonas maltophilia
1.1.1.169 NaAsO2 carbonyl reagent Stenotrophomonas maltophilia
1.1.1.169 NADP+ competitive with NADPH at unsaturation level of ketopantoic acid Stenotrophomonas maltophilia
1.1.1.169 p-chloromercuribenzoate sulfhydryl reagents, slightly inhibitory Stenotrophomonas maltophilia
1.1.1.169 phenylhydrazine carbonyl reagent Stenotrophomonas maltophilia
1.1.1.169 quercetin 12.0% at 0.1 mM Stenotrophomonas maltophilia
1.1.1.169 Semicarbazide carbonyl reagent Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.169 0.0318
-
 NADPH pH 7.0 Stenotrophomonas maltophilia
1.1.1.169 0.4
-
 ketopantoic acid pH 7.0 Stenotrophomonas maltophilia
1.1.1.169 8.55
-
 2-Keto-3-hydroxyisovalerate
-
 Stenotrophomonas maltophilia
1.1.1.169 52.1
-
 D-pantoic acid pH 8.0 Stenotrophomonas maltophilia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.169 additional information Mg2+ independent Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.169 30500
-
 3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia
1.1.1.169 87000
-
 +/-5000, sedimentation equilibrium method Stenotrophomonas maltophilia
1.1.1.169 115000
-
 +/-5000, gel filtration Stenotrophomonas maltophilia
1.1.1.169 140000 160000 +/-5000, HPLC gel filtration Stenotrophomonas maltophilia

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.169 Saccharomyces cerevisiae
-
-
-
1.1.1.169 Stenotrophomonas maltophilia
-
 845
-
1.1.1.169 Stenotrophomonas maltophilia 845
-
 845
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.169 gel chromatography Stenotrophomonas maltophilia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.169 633
-
 crystalline enzyme Stenotrophomonas maltophilia
1.1.1.169 669
-
-
 Stenotrophomonas maltophilia

Storage Stability

EC Number Storage Stability Organism
1.1.1.169 4°C, 0.01 M Tris/HCl buffer, pH 7.4, 0.1 mM DTT, 0.2 M NaCl, 6 months Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.169 2-keto-3-hydroxyisovalerate + NADPH
-
 Stenotrophomonas maltophilia ? + NADP+
-
 r
1.1.1.169 2-keto-3-hydroxyisovalerate + NADPH
-
 Stenotrophomonas maltophilia 845 ? + NADP+
-
 r
1.1.1.169 ketopantoic acid + NADPH
-
 Stenotrophomonas maltophilia D-pantoic acid + NADP+
-
 r
1.1.1.169 ketopantoic acid + NADPH
-
 Stenotrophomonas maltophilia 845 D-pantoic acid + NADP+
-
 r
1.1.1.169 additional information high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate Stenotrophomonas maltophilia ?
-
 ?
1.1.1.169 additional information high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate Stenotrophomonas maltophilia 845 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.169 oligomer 3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.169 37
-
 reduction of ketopantoic acid Stenotrophomonas maltophilia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.169 15 60 15°C: about 70% of activity maximum, 60°C: about 85% of activity maximum Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.169 30
-
 30 min, pH 6-10 Stenotrophomonas maltophilia
1.1.1.169 60
-
 10 min, stable below 60°C Stenotrophomonas maltophilia
1.1.1.169 70
-
 10 min, 70% loss of activity Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.169 6
-
 reduction of ketopantoic acid Stenotrophomonas maltophilia
1.1.1.169 8.5
-
 oxidation of D-pantoic acid Stenotrophomonas maltophilia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.169 6 10 30°C, 30 min Stenotrophomonas maltophilia
1.1.1.169 7
-
 stable below 60°C for 10 min Stenotrophomonas maltophilia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 additional information no activity with NADH Stenotrophomonas maltophilia
1.1.1.169 NADP+
-
 Stenotrophomonas maltophilia
1.1.1.169 NADPH
-
 Saccharomyces cerevisiae
1.1.1.169 NADPH specific Stenotrophomonas maltophilia