BRENDA - Enzyme Database

Primary alcohol dehydrogenases from Acinetobacter

Finnerty, W.R.; Methods Enzymol. 188, 14-18 (1990)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.164
highly unstable, more than 50% loss of activity within 24 h
Acinetobacter sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.164
rifampicin
inhibits enzyme induction in hexadecanol-grown cells, not in cells grown on ethanol, palmitate or hexadecane
Acinetobacter sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.164
0.003
-
hexadecanol
growth on ethanol, NAD+
Acinetobacter sp.
1.1.1.164
0.0073
-
hexadecanol
growth on palmitic acid, NAD+
Acinetobacter sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.164
hexadecanal + NADH
Acinetobacter sp.
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
-
-
1.1.1.164
hexadecanal + NADH
Acinetobacter sp. HO1-N
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.164
Acinetobacter sp.
-
-
-
1.1.1.164
Acinetobacter sp. HO1-N
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.164
partial
Acinetobacter sp.
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.164
-20°C, Tris-HCl 10 mM, pH 7.8, precipitation at 50% ammonium sulfate saturation, dissolved in 15fold reduced volume
Acinetobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286097
Acinetobacter sp.
hexadecanol + NAD+
-
-
-
-
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286097
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
-
-
-
1.1.1.164
additional information
enzyme is not visible as enzymatically active protein in zymograms
286097
Acinetobacter sp.
?
-
-
-
-
1.1.1.164
additional information
enzyme is not visible as enzymatically active protein in zymograms
286097
Acinetobacter sp. HO1-N
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.164
NAD+
preference for
Acinetobacter sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.164
NAD+
preference for
Acinetobacter sp.
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.164
highly unstable, more than 50% loss of activity within 24 h
Acinetobacter sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.164
rifampicin
inhibits enzyme induction in hexadecanol-grown cells, not in cells grown on ethanol, palmitate or hexadecane
Acinetobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.164
0.003
-
hexadecanol
growth on ethanol, NAD+
Acinetobacter sp.
1.1.1.164
0.0073
-
hexadecanol
growth on palmitic acid, NAD+
Acinetobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.164
hexadecanal + NADH
Acinetobacter sp.
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
-
-
1.1.1.164
hexadecanal + NADH
Acinetobacter sp. HO1-N
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.164
partial
Acinetobacter sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.164
-20°C, Tris-HCl 10 mM, pH 7.8, precipitation at 50% ammonium sulfate saturation, dissolved in 15fold reduced volume
Acinetobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286097
Acinetobacter sp.
hexadecanol + NAD+
-
-
-
-
1.1.1.164
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286097
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
-
-
-
1.1.1.164
additional information
enzyme is not visible as enzymatically active protein in zymograms
286097
Acinetobacter sp.
?
-
-
-
-
1.1.1.164
additional information
enzyme is not visible as enzymatically active protein in zymograms
286097
Acinetobacter sp. HO1-N
?
-
-
-
-