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Literature summary extracted from

  • Marshall, J.H.; Kong, Y.C.; Sloan, J.; May, J.W.
    Purification and properties of glycerol:NADP+ 2-oxidoreductase from Schizisaccharomyces pombe (1989), J. Gen. Microbiol., 135, 697-701.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.156 2-mercaptoethanol 80% inhibition at 1 mM Schizosaccharomyces pombe
1.1.1.156 8-hydroxyquinoline complete inhibition at 10 mM Schizosaccharomyces pombe
1.1.1.156 dithiothreitol 25% inhibition at 10 mM Schizosaccharomyces pombe
1.1.1.156 L-cysteine 40% inhibition at 10 mM Schizosaccharomyces pombe
1.1.1.156 p-chloromercuribenzoate strong inhibition Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.156 0.1
-
NADP+
-
Schizosaccharomyces pombe
1.1.1.156 0.34
-
NADPH
-
Schizosaccharomyces pombe
1.1.1.156 0.67
-
dihydroxyacetone
-
Schizosaccharomyces pombe
1.1.1.156 51
-
glycerol
-
Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.156 25000
-
1 * 30000 + 1 * 25000, SDS-PAGE Schizosaccharomyces pombe
1.1.1.156 30000
-
1 * 30000 + 1 * 25000, SDS-PAGE Schizosaccharomyces pombe
1.1.1.156 57000
-
gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.156 glycerol + NADP+ Schizosaccharomyces pombe
-
dihydroxyacetone + NADPH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.156 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.156 to homogeneity, 1step chromatography Schizosaccharomyces pombe

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.156 culture condition:dihydroxyacetone-grown cell
-
Schizosaccharomyces pombe
-
1.1.1.156 culture condition:glycerol-grown cell
-
Schizosaccharomyces pombe
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.156 15.7
-
-
Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.156 1,2,3-butanetriol + NADP+ 18% of the activity compared to glycerol Schizosaccharomyces pombe 1,3-dihydroxybutan-2-one + NADPH
-
?
1.1.1.156 1,2-butanediol + NADP+ 19% of the activity compared to glycerol Schizosaccharomyces pombe 1-hydroxybutan-2-one + NADPH
-
?
1.1.1.156 1,2-propanediol + NADP+ 30% of the activity compared to glycerol, low reverse reaction rate Schizosaccharomyces pombe hydroxyacetone + NADPH
-
r
1.1.1.156 2,3-butanediol + NADP+ 25% of the activity compared to glycerol, low reverse reaction rate Schizosaccharomyces pombe 3-hydroxybutan-2-one + NADPH
-
r
1.1.1.156 glycerol + NADP+
-
Schizosaccharomyces pombe dihydroxyacetone + NADPH + H+
-
r
1.1.1.156 propan-2-ol + NADP+ very low activity Schizosaccharomyces pombe acetone + NADPH
-
?
1.1.1.156 pyruvaldehyde + NADPH very low activity Schizosaccharomyces pombe lactaldehyde + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.156 dimer 1 * 30000 + 1 * 25000, SDS-PAGE Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
1.1.1.156 GDH4
-
Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.156 6
-
dihydroxyacetone reduction Schizosaccharomyces pombe
1.1.1.156 9.5
-
glycerol oxidation Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.156 NADP+ can not be replaced by NAD+ Schizosaccharomyces pombe