EC Number | General Stability | Organism |
---|---|---|
1.1.1.325 | stability of the purified enzyme disappears in sodium citrate buffer and potassium phosphate buffer for 24 h at 4°C, it increases continuously in Tris-HCl buffer and in glycine/NaOH buffer | Chlorobaculum tepidum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.153 | Melatonin | very slight | Chlorobaculum tepidum | |
1.1.1.153 | N-acetyldopamine | very slight | Chlorobaculum tepidum | |
1.1.1.153 | N-acetylserotonin | very slight | Chlorobaculum tepidum | |
1.1.1.325 | additional information | N-acetylserotonin and melatonin are no inhibitors | Chlorobaculum tepidum | |
1.1.1.325 | N-acetyldopamine | - |
Chlorobaculum tepidum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.153 | 0.0062 | - |
NADPH | - |
Chlorobaculum tepidum | |
1.1.1.153 | 0.021 | - |
sepiapterin | - |
Chlorobaculum tepidum | |
1.1.1.153 | 4 | - |
diacetyl | - |
Chlorobaculum tepidum | |
1.1.1.325 | 0.0062 | - |
NADPH | pH 8.8, 45°C | Chlorobaculum tepidum | |
1.1.1.325 | 0.021 | - |
sepiapterin | pH 8.8, 45°C | Chlorobaculum tepidum | |
1.1.1.325 | 4 | - |
diacetyl | pH 8.8, 45°C | Chlorobaculum tepidum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.325 | cytosol | - |
Chlorobaculum tepidum | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.153 | 26000 | - |
2 * 26000, gradient SDS-PAGE | Chlorobaculum tepidum |
1.1.1.153 | 55000 | - |
gel filtration | Chlorobaculum tepidum |
1.1.1.325 | 26000 | - |
2 * 26000, SDS-PAGE | Chlorobaculum tepidum |
1.1.1.325 | 55000 | - |
gel filtration | Chlorobaculum tepidum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.153 | 6-(S)-lactoyl-7,8-dihydropterin + NADPH | Chlorobaculum tepidum | - |
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
1.1.1.325 | 6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+ | Chlorobaculum tepidum | - |
L-threo-7,8-dihydrobiopterin + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.153 | Chlorobaculum tepidum | - |
- |
- |
1.1.1.325 | Chlorobaculum tepidum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.153 | - |
Chlorobaculum tepidum |
1.1.1.325 | native enzyme 533fold from cytosol by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by affinity chromatography, another different step of anion exchange chromatography, and ultrafiltration | Chlorobaculum tepidum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.153 | L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ | enzyme also reduces carbonyl compounds of non-pteridine derivatives | Chlorobaculum tepidum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.153 | 31.5 | - |
- |
Chlorobaculum tepidum |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.153 | -70°C, in 20 mM Tris-HCl, pH 8.8 | Chlorobaculum tepidum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.153 | 6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Chlorobaculum tepidum | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
1.1.1.153 | diacetyl + NADPH | - |
Chlorobaculum tepidum | ? | - |
? | |
1.1.1.153 | dihydrotepidopterin + NADP+ | 15% relative activity | Chlorobaculum tepidum | ? | - |
? | |
1.1.1.153 | L-erythro-dihydrobiopterin + NADP+ | - |
Chlorobaculum tepidum | ? | - |
? | |
1.1.1.153 | L-threo-dihydrobiopterin + NADP+ | D-isomer 45% relative activity | Chlorobaculum tepidum | ? | - |
r | |
1.1.1.153 | L-threo-dihydroneopterin + NADP+ | - |
Chlorobaculum tepidum | ? | - |
? | |
1.1.1.153 | additional information | not: methylglyoxal, menadione | Chlorobaculum tepidum | ? | - |
? | |
1.1.1.153 | phenylglyoxal + NADPH | - |
Chlorobaculum tepidum | ? | - |
? | |
1.1.1.325 | 6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+ | - |
Chlorobaculum tepidum | L-threo-7,8-dihydrobiopterin + NADP+ | - |
? | |
1.1.1.325 | diacetyl + NADPH + H+ | - |
Chlorobaculum tepidum | ? + NADP+ | - |
? | |
1.1.1.325 | L-threo-7,8-dihydrobiopterin + NADP+ | - |
Chlorobaculum tepidum | sepiapterin + NADPH + H+ | - |
r | |
1.1.1.325 | additional information | the enzyme also shows activity with D-threo-dihydrobiopterin, L-threo-dihydroneopterin, and dihydrotepidopterin, and low activity with L-erythro-dihydrobiopterin | Chlorobaculum tepidum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.153 | homodimer | 2 * 26000, gradient SDS-PAGE | Chlorobaculum tepidum |
1.1.1.325 | homodimer | 2 * 26000, SDS-PAGE | Chlorobaculum tepidum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.325 | sepiapterin reductase | - |
Chlorobaculum tepidum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.153 | 50 | - |
highest activity, activity decreases slowly above | Chlorobaculum tepidum |
1.1.1.325 | 50 | - |
- |
Chlorobaculum tepidum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.153 | 25 | 50 | relativley stable for 2 h in this range, loss of activity in 20 min at 70°C | Chlorobaculum tepidum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.325 | 5 | - |
NADPH | pH 8.8, 45°C | Chlorobaculum tepidum | |
1.1.1.325 | 5 | - |
sepiapterin | pH 8.8, 45°C | Chlorobaculum tepidum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.153 | 8.8 | - |
in Tris-HCl buffer or in glycine-NaOH buffer | Chlorobaculum tepidum |
1.1.1.325 | 8.8 | - |
- |
Chlorobaculum tepidum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.153 | additional information | does not show any activity with NADH | Chlorobaculum tepidum | |
1.1.1.325 | NADPH | - |
Chlorobaculum tepidum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.325 | 0.4 | - |
N-acetyldopamine | pH 8.8, 45°C | Chlorobaculum tepidum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.325 | additional information | alignment of amino acid sequences near the N-termini of sepiapterin reductases from various sources, overview | Chlorobaculum tepidum |