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Literature summary extracted from
- Sepiapterin reductase exhibits a NADPH-dependent dicarbonyl reductase activity (1984), Biochem. Biophys. Res. Commun., 118, 859-866.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.153 | 0.0143 | - |
2-amino-6-(2-hydroxypropanoyl)pteridin-4(1H)-one | pH 6.4, 25°C | Rattus norvegicus |  |
| 1.1.1.153 | 0.02 | - |
benzil | pH 6.4, 25°C | Rattus norvegicus | |
| 1.1.1.153 | 0.0218 | - |
phenylpropanedione | - |
Rattus norvegicus | |
| 1.1.1.153 | 0.0218 | - |
phenylpropanedione | pH 6.4, 25°C | Rattus norvegicus | |
| 1.1.1.153 | 1 | - |
diacetyl | - |
Rattus norvegicus | |
| 1.1.1.153 | 1 | - |
diacetyl | pH 6.4, 25°C | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.153 | 6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | - |
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
| 1.1.1.153 | sepiapterin + NADPH + H+ | Rattus norvegicus | i.e. 2-amino-4-hydroxy-6-lactyl-7,8-dihydropteridine, biosynthesis pathway, overview | 7,8-dihydrobiopterin + NADP+ | i.e. 2-amino-4-hydroxy-6-(1',2'-dihydroxypropyl-)7,8-dihydropteridine, which is a substrate for biosynthesis of tetrahydrobiopterin, i.e. 6R-L-erythro-1',2'-dihydroxy-propyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine, an important component for controling the concentration of catecholamines or indoleamines in the brain and other nerve tissues | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.153 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.153 | - |
Rattus norvegicus |
| 1.1.1.153 | native enzyme from erythrocytes | Rattus norvegicus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.153 | L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ | enzyme also reduces carbonyl compounds of non-pteridine derivatives | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.153 | brain | - |
Rattus norvegicus | - |
| 1.1.1.153 | erythrocyte | - |
Rattus norvegicus | - |
| 1.1.1.153 | nerve | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.153 | additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.153 | 1-phenyl-1,2-propanedione + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | 2,3-butanediol + NADP+ | - |
Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | 2-amino-6-(2-hydroxypropanoyl)pteridin-4(1H)-one + NADPH + H+ | - |
Rattus norvegicus | ? | - |
r | |
| 1.1.1.153 | 6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
| 1.1.1.153 | 9,10-phenanthrenequinone + NADP+ | - |
Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | acetoin + NADPH | 0.003 mM NADPH oxidised per 30 min | Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | benzil + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | benzil + NADPH | NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | diacetyl + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | diacetyl + NADPH | NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus | acetoin + NADP+ | - |
? | |
| 1.1.1.153 | additional information | some monoketotestosterones show slight activity | Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | additional information | substrate specificity, the enzyme also exhibits non-specific NADPH-dependent dicarbonyl reductase activity, overview | Rattus norvegicus | ? | - |
? | |
| 1.1.1.153 | phenylpropanedione + NADPH | NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus | phenylpropan-2,3-diol + NADP+ | - |
? | |
| 1.1.1.153 | sepiapterin + NADPH + H+ | i.e. 2-amino-4-hydroxy-6-lactyl-7,8-dihydropteridine, biosynthesis pathway, overview | Rattus norvegicus | 7,8-dihydrobiopterin + NADP+ | i.e. 2-amino-4-hydroxy-6-(1',2'-dihydroxypropyl-)7,8-dihydropteridine, which is a substrate for biosynthesis of tetrahydrobiopterin, i.e. 6R-L-erythro-1',2'-dihydroxy-propyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine, an important component for controling the concentration of catecholamines or indoleamines in the brain and other nerve tissues | ? | |
| 1.1.1.153 | sepiapterin + NADPH + H+ | sepiapterin reductase activity | Rattus norvegicus | 7,8-dihydrobiopterin + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.153 | SPR | - |
Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.153 | 25 | - |
assay at, sepiapterin reductase activity | Rattus norvegicus |
| 1.1.1.153 | 37 | - |
assay at, NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.153 | 6.4 | - |
assay at, sepiapterin reductase activity | Rattus norvegicus |
| 1.1.1.153 | 6.8 | - |
assay at, NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.153 | NADPH | - |
Rattus norvegicus | |
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