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Literature summary extracted from

  • Roe, C.R.; Kaplan, N.O.
    Purification and substrate specificities of bacterial hydroxysteroid dehydrogenases (1969), Biochemistry, 8, 5093-5103.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.152 analysis selective microquantitation of steroid substrates Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.152 47000
-
 meniscus depletion method, gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.152 3alpha-hydroxy-5beta-androstane-17-one + NAD+ Comamonas testosteroni etiocholanolone, strong substrate specificity 5beta-androstane-3,17-dione + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.152 Comamonas testosteroni
-
 2 strains, ATCC 11996 and STDH-m
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.152 to homogeneity, chromatography techniques, preparative gel electrophoresis Comamonas testosteroni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.152 cell culture
-
 Comamonas testosteroni
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.152 3alpha-hydroxy-5beta-androstane-17-one + NAD+ etiocholanolone, strong substrate specificity Comamonas testosteroni 5beta-androstane-3,17-dione + NADH
-
 ir

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.152 NAD+
-
 Comamonas testosteroni