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Literature summary extracted from

  • Ma, H.; Penning, T.M.
    Conversion of mammalian 3alpha-hydroxysteroid dehydrogenase to 20alpha-hydroxysteroid dehydrogenase using loop chimeras: changing specificity from androgens to progestins (1999), Proc. Natl. Acad. Sci. USA, 96, 11161-11166.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.149 subcloned, overexpressed in Escherichia coli DH5alpha or C41 (DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.149 additional information loops of 3alpha-hydroxysteroid dehydrogenase exchanged by PCR generate 20alpha-hydroxysteroid dehydrogenase, loops A, B and C yields total 20alpha-hydroxysteroid dehydrogenase activity, replacement of A and C yields bifunctional enzyme, several point mutations in the pocket of 3alpha-hydroxysteroid dehydrogenase show no 20alpha-hydroxysteroid dehydrogenase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.0075
-
 20alpha-hydroxyprogesterone
-
 Rattus norvegicus
1.1.1.149 0.12
-
 20alpha-hydroxyprogesterone A, B and C loop changed, total converted enzyme Rattus norvegicus
1.1.1.149 0.13
-
 20alpha-hydroxyprogesterone A and C loop changed, bifunctional enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 anion exchange and affinity chromatography Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.149 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ bibi mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 20alpha-hydroxyprogesterone + NADP+
-
 Rattus norvegicus progesterone + NADPH
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.149 0.04
-
 20alpha-hydroxyprogesterone +/-0.4, A, B and C loop changed, totally converted enzyme Rattus norvegicus
1.1.1.149 0.055
-
 20alpha-hydroxyprogesterone +/-0.4, A and C loop changed, bifunctional enzyme Rattus norvegicus
1.1.1.149 1.06
-
 20alpha-hydroxyprogesterone +/-0.3 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADP+
-
 Rattus norvegicus
1.1.1.149 NADPH
-
 Rattus norvegicus