Literature summary extracted from

Albarracin, C.T.; Parmer, T.G.; Duan, W.R.; Nelson, S.E.; Gibori, G.
Identification of a major prolactin-regulated protein as 20alpha-hydroxysteroid dehydrogenase: coordinate regulation of its activity, protein content, and messenger ribonucleic acid expression (1994), Endocrinology, 134, 2453-2460.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.149	additional information	no inhibition but increase at preincubation with antiserum	Rattus norvegicus
1.1.1.149	prolactin	down-regulating of gene-expression	Rattus norvegicus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.149	37000	-	SDS-PAGE	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.149	Rattus norvegicus	-	female, pregnant, Sprague-Dawley	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
1.1.1.149	phosphoprotein	-	Rattus norvegicus

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.149	solubilized, separated by SDS-PAGE, protein extraction from gel, renatured	Rattus norvegicus

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
1.1.1.149	6 M guanidine hydrochloride, 20 mM Tris-HCl buffer, pH 7.4, 100 mM KCl, 10% glycerol, 1 mM DTT, 1 h, 25°C	Rattus norvegicus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.149	ovary	corpus luteum	Rattus norvegicus	-

Storage Stability

EC Number	Storage Stability	Organism
1.1.1.149	-80°C, particulate and cytosolic fraction after homogenization	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.149	20alpha-dihydroprogesterone + NADP+	-	Rattus norvegicus	progesterone + NADPH	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.149	monomer	1 * 37000, SDS-PAGE	Rattus norvegicus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.1.1.149	95	-	15 min, almost total loss of activity	Rattus norvegicus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.149	NADP+	-	Rattus norvegicus
1.1.1.149	NADPH	-	Rattus norvegicus

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Release 2023.1 (January 2023)