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Literature summary extracted from

  • Ma, H.; Penning, T.M.
    Characterization of homogeneous recombinant rat ovarian 20alpha-hydroxysteroid dehydrogenase: fluorescent properties and inhibition profile (1999), Biochem. J., 341, 853-859.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.149 expressed in Escherichia coli C41 (DE3) Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.149 Flufenamic acid poor inhibition for 20alpha-hydroxyprogesterone oxidation Rattus norvegicus
1.1.1.149 Meclofenamic acid 20alpha-hydroxyprogesterone oxidation Rattus norvegicus
1.1.1.149 additional information not inhibited by indomethacin, tolmetin and steroidal anti-inflammatory drugs Rattus norvegicus
1.1.1.149 synthetic non-steroidal oestrogens hexoestrol most potent competitive inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.00195
-
 9,10-phenanthrenequinone +/-0.0004 Rattus norvegicus
1.1.1.149 0.008
-
 NADP+ +/-0.0011 Rattus norvegicus
1.1.1.149 0.009
-
 20alpha-hydroxyprogesterone +/-0.0001 Rattus norvegicus
1.1.1.149 0.011
-
 progesterone +/-0.0017 Rattus norvegicus
1.1.1.149 0.012
-
 3alpha-hydroxy-5alpha-pregnan-20-one +/-0.0035 Rattus norvegicus
1.1.1.149 0.013
-
 20alpha-hydroxy-5alpha-pregnan-3-one +/-0.0035 Rattus norvegicus
1.1.1.149 0.014
-
 NADPH +/-0.004 Rattus norvegicus
1.1.1.149 0.0154
-
 17alpha-hydroxyprogesterone +/-0.0029 Rattus norvegicus
1.1.1.149 0.3811
-
 4-nitrobenzaldehyde +/-0.0605 Rattus norvegicus
1.1.1.149 120
-
 D,L-glyceraldehyde
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 37000
-
 SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Rattus norvegicus
-
 female, Sprague-Dawley, 2 weeks
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 anion exchange and affinity chromatography Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.149 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ bibi mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 ovary
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.149 0.002
-
 reduction of 0.06 mM prostaglandin E2 Rattus norvegicus
1.1.1.149 0.007
-
 reduction of 0.03 mM 17alpha-hydroxy-pregnenolone Rattus norvegicus
1.1.1.149 0.013
-
 reduction of 100 mM D-glucose Rattus norvegicus
1.1.1.149 0.016
-
 reduction of 0.03 mM prednisolone Rattus norvegicus
1.1.1.149 0.017
-
 reduction of 100 mM D-galactose Rattus norvegicus
1.1.1.149 0.025
-
 reduction of 0.03 mM prednisone Rattus norvegicus
1.1.1.149 0.067
-
 reduction of 0.05 mM pregnenolone Rattus norvegicus
1.1.1.149 0.9
-
 reduction of 0.06 mM 5alpha-pregnane-3,20-dione Rattus norvegicus
1.1.1.149 2.09
-
 20alpha-hydroxyprogesterone Rattus norvegicus
1.1.1.149 2.5
-
 oxidation of 0.06 mM 20alpha-hydroxy-5beta-pregnan-3-one Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.149 -70°C, 2.8 mg/ml protein concentration, 20 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 1 mM beta-mercaptoethanol, 30%, v/v, glycerol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 17alpha-hydroxypregnenolone + NADPH
-
 Rattus norvegicus ? + NADP+
-
 ?
1.1.1.149 17alpha-hydroxyprogesterone + NADPH + H+
-
 Rattus norvegicus 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+
-
 ?
1.1.1.149 20alpha-hydroxy-5alpha-pregnan-3-one + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-20alpha,3alpha-diol + NADP+
-
 r
1.1.1.149 3alpha-hydroxy-5-alpha-pregnan-20-one + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-3alpha,20alpha-diol + NADP+
-
 r
1.1.1.149 3alpha-hydroxy-5alpha-pregnan-20-one + NADPH + H+
-
 Rattus norvegicus 5alpha-pregnan-3alpha,20alpha-diol + NADP+
-
 r
1.1.1.149 4-nitrobenzaldehyde + NADPH
-
 Rattus norvegicus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.149 9,10-phenanthrenequinone + NADPH
-
 Rattus norvegicus NADP+ + ?
-
 ?
1.1.1.149 DL-glyceraldehyde + NADPH
-
 Rattus norvegicus glycerol + NADP+
-
 ?
1.1.1.149 additional information high activity for neuroactive steroids, 9,10-phenanthrenequinone and 4-nitrobenzaldehyde, low activity for 17alpha hydroxypregnenolone, D,L-glyceraldehyde and prostaglandin E2, no activity for 20beta-hydroxy-pregn-4-en-3-one, andro-, testosterone, 17alpha-, 17beta-oestradiol, oestrone and 5alpha-dihydrotestosterone, other substrates: DL-glyceraldehyde, prostaglandin E2, D-glucose, prednisolone, D-galactose, prednisone, pregnenolone, 5alpha-pregnane-3,20-dione, 9,10-phenanthrenequinone Rattus norvegicus ?
-
 ?
1.1.1.149 progesterone + NADPH
-
 Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
 r
1.1.1.149 prostaglandin E2 + NADPH low activity Rattus norvegicus NADP+ + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.149 monomer 1 * 37000, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.149 0.178
-
 17alpha-hydroxyprogesterone +/-0.8 Rattus norvegicus
1.1.1.149 0.208
-
 3alpha-hydroxy-5-alpha-pregnan-20-one +/-1.6 Rattus norvegicus
1.1.1.149 0.215
-
 D,L-glyceraldehyde +/-0.3 Rattus norvegicus
1.1.1.149 0.785
-
 20alpha-hydroxy-5alpha-pregnan-3-one +/-4.3 Rattus norvegicus
1.1.1.149 1.17
-
 20alpha-hydroxyprogesterone +/-0.2 Rattus norvegicus
1.1.1.149 1.43
-
 NADPH +/-1.3 Rattus norvegicus
1.1.1.149 1.52
-
 NADP+ +/-6.1 Rattus norvegicus
1.1.1.149 1.65
-
 progesterone +/-6.7 Rattus norvegicus
1.1.1.149 5.91
-
 4-nitrobenzaldehyde +/-33.4 Rattus norvegicus
1.1.1.149 7.7
-
 9,10-phenanthrenequinone +/-26.9 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADP+ strictly specific Rattus norvegicus
1.1.1.149 NADPH strictly specific Rattus norvegicus