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Literature summary extracted from

  • Nakajin, S.; Kawai, Y.; Ohno, S.; Shinoda, M.
    Purification and characterization of pig adrenal 20alpha-hydroxysteroid dehydrogenase (1989), J. Steroid Biochem., 33, 1181-1189.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.149
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.0262
-
 17alpha-hydroxyprogesterone isozyme HSD-I Sus scrofa
1.1.1.149 0.118
-
 17alpha-hydroxyprogesterone isozyme HSD-II Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 30000
-
 isozyme HSD-I, HSD-II, gel filtration Sus scrofa
1.1.1.149 39000
-
 isozyme HSD-I, SDS-PAGE Sus scrofa
1.1.1.149 40000
-
 isozyme HSD-II, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.149 glycoprotein both isozymes Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 ammonium sulfate precipitation, affinity chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 adrenal gland
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 17alpha-hydroxyprogesterone + NAD(P)H + H+ NADPH preferred Sus scrofa 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.149 monomer 1 * 39000 isozyme HSD-I, 1 * 40000 isozyme HSD-I, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADH
-
 Sus scrofa
1.1.1.149 NADPH preferred Sus scrofa