Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mori, M.; Wiest, W.G.
    Purification of rat ovary 20alpha-hydroxysteroid dehydrogenase by affinity chromatography (1979), J. Steroid Biochem., 11, 1443-1449.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.149
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Rattus norvegicus
-
 young, pregnant, female
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 ion exchange chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 ovary
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.149 8.318
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.149 4°C, cytosol preparation prior to purification, several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 20alpha-hydroxyprogesterone + NADP+
-
 Rattus norvegicus progesterone + NADPH
-
 ?
1.1.1.149 additional information no activity with 20beta-hydroxypregn-4-en-3-one, 5alpha-pregnan-3,20-dione, 3beta-hydroxy-5alpha-pregnan-20-one and 3alpha,20alpha-dihydroxy-5alpha-pregnane Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.149 monomer
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADP+
-
 Rattus norvegicus