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Literature summary extracted from

  • Noda, K.; Shiota, K.; Takahashi, M.
    Purification and characterization of rat ovarian 20alpha-hydroxysteroid dehydrogenase (1991), Biochim. Biophys. Acta, 1079, 112-118.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.149 isozyme HSD1, HSD2 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.00087
-
 NADP+ HSD2 Rattus norvegicus
1.1.1.149 0.00105
-
 NADP+ HSD1 Rattus norvegicus
1.1.1.149 0.00475
-
 20alpha-hydroxy-4-pregnen-3-one HSD1 Rattus norvegicus
1.1.1.149 0.00516
-
 20alpha-hydroxy-4-pregnen-3-one HSD2 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 33000
-
 1 * 33000, SDS-PAGE Rattus norvegicus
1.1.1.149 41000
-
 gel filtration, non-reducing conditions Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Rattus norvegicus
-
 female, Wistar-Imamichi strain
-
1.1.1.149 Rattus norvegicus Wistar-Imamichi
-
 female, Wistar-Imamichi strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 isozyme HSD1 and HSD2, anion-exchange and affinity chromatography, capillary electrophoresis Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 ovary
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.149 0.2769
-
 HSD2, reduction of 17alpha-hydroxyprogesterone Rattus norvegicus
1.1.1.149 1.462
-
 HSD1, reduction of 17alpha-hydroxyprogesterone Rattus norvegicus
1.1.1.149 3.815
-
 HSD2, reduction of progesterone Rattus norvegicus
1.1.1.149 4.618
-
 HSD1 Rattus norvegicus
1.1.1.149 6.515
-
 HSD2 Rattus norvegicus
1.1.1.149 8.154
-
 HSD1, reduction of progesterone Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 17alpha-hydroxyprogesterone + NADPH
-
 Rattus norvegicus 17alpha,20alpha-dihydroxyprogesterone + NADP+
-
 ?
1.1.1.149 17alpha-hydroxyprogesterone + NADPH
-
 Rattus norvegicus Wistar-Imamichi 17alpha,20alpha-dihydroxyprogesterone + NADP+
-
 ?
1.1.1.149 additional information no activity with corticosterone and 5alpha-dihydrotestosterone as substrates, other substrate: 17alpha-hydroxyprogesterone Rattus norvegicus ?
-
 ?
1.1.1.149 additional information no activity with corticosterone and 5alpha-dihydrotestosterone as substrates, other substrate: 17alpha-hydroxyprogesterone Rattus norvegicus Wistar-Imamichi ?
-
 ?
1.1.1.149 progesterone + NADPH
-
 Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
 r
1.1.1.149 progesterone + NADPH
-
 Rattus norvegicus Wistar-Imamichi 20alpha-hydroxyprogesterone + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.149 monomer 1 * 33000, SDS-PAGE Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADP+
-
 Rattus norvegicus
1.1.1.149 NADPH
-
 Rattus norvegicus