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Literature summary extracted from

  • Strickler, R.C.; Tobias, B.; Covey, D.F.
    Human placental 17beta-estradiol dehydrogenase and 20alpha-hydroxysteroid dehydrogenase. Two activities at a single enzyme active site (1981), J. Biol. Chem., 256, 316-321.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.149 16alpha-Bromoacetoxyprogesterone
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.62 0.0086
-
 estrone
-
 Homo sapiens
1.1.1.149 0.2
-
 progesterone
-
 Homo sapiens
1.1.1.149 2.5
-
 20alpha-hydroxyprogesterone
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.62 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 35000
-
 1 * 35000, SDS-PAGE Homo sapiens
1.1.1.149 35000
-
 2 * 35000, SDS disc gel electrophorese Homo sapiens
1.1.1.149 68000
-
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.62 Homo sapiens
-
 17beta,20alpha-hydroxysteroid dehydrogenase
-
1.1.1.149 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.62 copurification of 17beta-estradiol dehydrogenase and 20alpha-hydroxysteroid dehydrogenase Homo sapiens
1.1.1.149 co-purification with 17beta-estradiol dehydrogenase, EC 1.1.1.62, affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.62 placenta
-
 Homo sapiens
-
1.1.1.149 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.149 0.038 0.046 calculated from 17beta-estadiol dehydrogenase activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.62 20alpha-hydroxy-4-pregnen-3-one + NADP+ low activity Homo sapiens 4-pregnen-3,20-dione + NADPH
-
 ?
1.1.1.62 estradiol-17beta + NAD+
-
 Homo sapiens estrone + NADH
-
 r
1.1.1.62 estrone + NADH
-
 Homo sapiens estradiol-17beta + NAD+
-
 r
1.1.1.62 progesterone + NADPH low activity Homo sapiens ? + NADP+
-
 ?
1.1.1.149 progesterone + NAD(P)H
-
 Homo sapiens 20alpha-hydroxyprogesterone + NAD(P)+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.149 dimer 2 * 35000, SDS disc gel electrophorese Homo sapiens
1.1.1.149 monomer 1 * 35000, SDS-PAGE Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.62 NAD+ cofactor Homo sapiens
1.1.1.62 NADH cofactor Homo sapiens
1.1.1.149 NADP+
-
 Homo sapiens
1.1.1.149 NADPH
-
 Homo sapiens