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Literature summary extracted from

  • De Prada, P.; Setchell, K.D.R.; Hylemon, P.B.
    Purification and characterization of a novel 17alpha-hydroxysteroid dehydrogenase from an intestinal Eubacterium sp. VPI 12708 (1994), J. Lipid Res., 35, 922-929.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.148 sensitive to molecular oxygen Eubacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.148 EDTA
-
 Eubacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.148 0.002
-
 NADP+
-
 Eubacterium sp.
1.1.1.148 0.005
-
 NADPH
-
 Eubacterium sp.
1.1.1.148 0.031
-
 4-androsten-3,17-dione
-
 Eubacterium sp.
1.1.1.148 0.07
-
 Epitestosterone
-
 Eubacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.148 Mg2+
-
 Eubacterium sp.
1.1.1.148 Zn2+
-
 Eubacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.148 42000
-
 4 * 42000, SDS-PAGE Eubacterium sp.
1.1.1.148 160000
-
 gel filtration Eubacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.148 Eubacterium sp.
-
 inducible by growth on androstenedione and cholic acid, not deoxycholic acid
-
1.1.1.148 Eubacterium sp.
-
 VPI 12708
-
1.1.1.148 Eubacterium sp. VPI 12708
-
 VPI 12708
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.148
-
 Eubacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.148 1.8
-
 oxidation of 17alpha-hydroxy-4-androsten-3-one Eubacterium sp.
1.1.1.148 3.25
-
 reduction of 4-androsten-3,17-dione Eubacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.148 17alpha-estradiol + NAD(P)+
-
 Eubacterium sp. estrone + NAD(P)H
-
 r
1.1.1.148 17alpha-estradiol + NAD(P)+
-
 Eubacterium sp. VPI 12708 estrone + NAD(P)H
-
 r
1.1.1.148 17alpha-hydroxy-4-androsten-3-one + NADP+ epitestosterone Eubacterium sp. 4-androsten-3,17-dione + NADPH
-
 r
1.1.1.148 17alpha-hydroxy-4-androsten-3-one + NADP+ epitestosterone Eubacterium sp. VPI 12708 4-androsten-3,17-dione + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.148 tetramer 4 * 42000, SDS-PAGE Eubacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.148 17alpha-hydroxysteroid dehydrogenase
-
 Eubacterium sp.
1.1.1.148 More N-terminal sequence comparison of several enzymes: 17alpha-hydroxysteroid dehydrogenase belongs to the disulfide oxidoreductase family, subfamily dihydrolipoamide dehydrogenases Eubacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.148 5.5 7 reduction of 4-androsten-3,17-dione Eubacterium sp.
1.1.1.148 6.5
-
 oxidation of 17alpha-hydroxy-4-androsten-3-one Eubacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.148 NADP+ highly specific for Eubacterium sp.