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Literature summary extracted from

  • Meigs, R.A.; Ryan, K.J.
    16alpha-Hydroxysteroid dehydrogenase of rat kidney. Purification, assay, and properties (1966), J. Biol. Chem., 241, 4011-4015.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.147 extremely unstable, glycerol partially protects against inactivation Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.147 1,10-phenanthroline complete inhibition at 0.1 mM Rattus sp.
1.1.1.147 2,2'-bipyridine 19% inhibition at 0.1 mM Rattus sp.
1.1.1.147 2-Iodoacetamide 86% inhibition at 1 mM Rattus sp.
1.1.1.147 8-hydroxy-5-quinolinesulfonate 89% inhibition at 1 mM Rattus sp.
1.1.1.147 Ag+ 78% inhibition at 0.01 mM Rattus sp.
1.1.1.147 Cu2+ 98% inhibition at 0.01 mM Rattus sp.
1.1.1.147 EDTA 97% inhibition at 0.01 mM Rattus sp.
1.1.1.147 N-ethylmaleimide 94% inhibition at 0.1 mM Rattus sp.
1.1.1.147 o-Iodosobenzoate complete inhibition at 0.1 mM Rattus sp.
1.1.1.147 p-hydroxymercuribenzoate complete inhibition at 0.01 mM Rattus sp.
1.1.1.147 Zn2+ 84% inhibition at 0.1 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.147 0.041
-
 NADPH
-
 Rattus sp.
1.1.1.147 0.07
-
 17alpha-estradiol-16-one
-
 Rattus sp.
1.1.1.147 0.083
-
 NADH
-
 Rattus sp.
1.1.1.147 0.089
-
 17beta-estradiol-16-one
-
 Rattus sp.
1.1.1.147 0.28
-
 16-ketotestosterone
-
 Rattus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.147 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.147 partial Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.147 kidney inducible by estrogen administration Rattus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.147 16-ketotestosterone + NAD(P)H
-
 Rattus sp. 16alpha-hydroxytestosterone + NAD(P)+
-
 r
1.1.1.147 16-oxo-17beta-estradiol + NADH + H+
-
 Rattus sp. 16alpha-hydroxy-17beta-estradiol + NAD+
-
 r
1.1.1.147 17alpha-estradiol-16-one + NAD(P)H best substrate tested, changing configuration or oxidation state of the neighboring oxygen substituent at C-17 increases the rate of reduction Rattus sp. 16alpha-hydroxy-17alpha-estradiol + NAD(P)+
-
 r
1.1.1.147 17beta-estradiol-16-one + NAD(P)H
-
 Rattus sp. 16alpha-hydroxy-17beta-estradiol + NAD(P)+
-
 r
1.1.1.147 3-hydroxy-estra-1,3,5(10)-triene-16,17-dione + NAD(P)H + H+
-
 Rattus sp. 3,16alpha-dihydroxy-estra-1,3,5(10)-triene-17-one + NAD(P)+
-
 r
1.1.1.147 3beta,17beta-dihydroxy-androst-5-en-16-one + NAD(P)H low activity Rattus sp. 3beta,16alpha,17beta-trihydroxyandrost-5-ene + NAD(P)+
-
 ?
1.1.1.147 estra-1,3,5(10)-triene-3,16beta,17beta-triol + NAD(P)+
-
 Rattus sp. estra-1,3,5(10)-triene-3,17beta-diol-16-one + NAD(P)H
-
 ?
1.1.1.147 estra-1,3,5(10)triene-3,17-beta-diol-16-one + NAD(P)H low activity Rattus sp. estra-1,3,5(10)triene-3,16alpha,17-triol + NAD(P)+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.147 6.1 6.2
-
 Rattus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.147 NADH 40% lower reaction rate than NADPH Rattus sp.
1.1.1.147 NADPH 40% greater reaction rate than NADH Rattus sp.