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Literature summary extracted from

  • Agarwal, A.K.; Tusie-Luna, M.T.; Monder, C.; White, P.C.
    Expression of 11beta-hydroxysteroid dehydrogenase using recombinant vaccinia virus (1990), Mol. Endocrinol., 4, 1827-1832.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.146 Triton DF-18 slight activation Rattus sp.
1.1.1.146 Triton X-100 slight activation Rattus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.146 expression in TK-143 B cells by vaccinia virus infection Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.146 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate decreased dehydrogenase activity, no effect on reductase activity; i.e. CHAPS Rattus sp.
1.1.1.146 3beta-hydroxy-11-oxo-18beta-olean-12-en-30-oic-acid 50% inhibition of dehydrogenase activity at 5 nM, 50% inhibition of oxoreductase activity at 20 nM; i.e. glycyrrhetinic acid Rattus sp.
1.1.1.146 A1-tunicamycin 50% inhibition at 50 ng/ml, reductase activity not affected Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.146 0.0011
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 7.0 Rattus sp.
1.1.1.146 0.00144
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 7.0 Rattus sp.
1.1.1.146 0.00214
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 8.5 Rattus sp.
1.1.1.146 0.00283
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 8.5 Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.146 11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ Rattus sp.
-
 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.146 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.146 glycoprotein
-
 Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.146 to homogeneity, recombinant enzyme Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.146 liver
-
 Rattus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.146 11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+
-
 Rattus sp. 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.146 37
-
 pH 7.0 or 8.5, stable for 1 h Rattus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.146 0.0000883
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 7.0 Rattus sp.
1.1.1.146 0.000113
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 7.0 Rattus sp.
1.1.1.146 0.000183
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 8.5 Rattus sp.
1.1.1.146 0.000228
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 8.5 Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.146 5.5 6.5 reductase activity Rattus sp.
1.1.1.146 8.5 9.5 oxidase activity Rattus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.146 7
-
 11-dehydrogenase activity stable for 2 h, reductase activity rapidly decreases Rattus sp.
1.1.1.146 8.5
-
 11-dehydrogenase activity stable for 2 h, reductase activity rapidly decreases Rattus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.146 NADP+
-
 Rattus sp.
1.1.1.146 NADPH
-
 Rattus sp.