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Literature summary extracted from

  • Jabarak, J.; Braithwaite, S.S.
    Kinetic studies on a 15-hydroxyprostaglandin dehydrogenase from human placenta (1976), Arch. Biochem. Biophys., 177, 245-254.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.141 NAD+ stabilizes Homo sapiens
1.1.1.141 no stabilization by addition of prostaglandins Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.141 13-cis-Prostaglandin F2alpha 0.124 mM, 50% inhibition Homo sapiens
1.1.1.141 15-epi-prostaglandin E1
-
 Homo sapiens
1.1.1.141 15-Epiprostaglandin E1 0.17 mM, 50% inhibition Homo sapiens
1.1.1.141 7-Oxa-13-prostynoic acid 0.0475 mM, 50% inhibition Homo sapiens
1.1.1.141 7-oxa-prostanoic acid
-
 Homo sapiens
1.1.1.141 7-Oxoprostanoic acid
-
 Homo sapiens
1.1.1.141 7-Thia-13-prostynoic acid 0.0068 mM, 50% inhibition Homo sapiens
1.1.1.141 7-thia-prostanoic acid
-
 Homo sapiens
1.1.1.141 arachidonic acid 0.0027 mM, 50% inhibition; irreversible inactivation Homo sapiens
1.1.1.141 ent-13-dehydro-15-epi-prostaglandin F2alpha
-
 Homo sapiens
1.1.1.141 Ent-13-dehydro-15-epiprostaglandin F3alpha 0.055 mM, 50% inhibition Homo sapiens
1.1.1.141 ent-13-dehydroprostaglandin E2 0.0068 mM, 50% inhibition Homo sapiens
1.1.1.141 epi-7-thia-prostaglandin F2alpha
-
 Homo sapiens
1.1.1.141 epi-7-thiaprostaglandin F2alpha 0.0052 mM, 50% inhibition Homo sapiens
1.1.1.141 Ethacrynic acid 0.0055 mM, 50% inhibition; irreversible inactivation Homo sapiens
1.1.1.141 additional information IC50 values at pH 7.0 and at pH 9.0; pH-dependent inhibition and inhibition mechanism, at high substrate concentrations there is formation of unreactive complexes between the 15-hydroxyrostaglandin and both the free enzyme and enzyme-NADH complex and between the 15-ketoprostaglandin and both the free enzyme and enzyme NAD+ complex Homo sapiens
1.1.1.141 nat-13-dehydroprostaglandin E2
-
 Homo sapiens
1.1.1.141 nat-13-dehydroprostaglandin F3alpha
-
 Homo sapiens
1.1.1.141 Nat-13-thiaprostaglandin F2alpha 0.0088 mM, 50% inhibition Homo sapiens
1.1.1.141 nat-7-thia-prostaglandin F2alpha
-
 Homo sapiens
1.1.1.141 nat-l3-dehydroprostaglandin F2alpha
-
 Homo sapiens
1.1.1.141 Prostaglandin B1 0.0497 mM, 50% inhibition Homo sapiens
1.1.1.141 Prostanoic acid 0.013 mM, 50% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.141 additional information
-
 additional information Michaelis-Menten kinetics, overview Homo sapiens
1.1.1.141 0.0013
-
 prostaglandin E1 at pH 7.0 Homo sapiens
1.1.1.141 0.0025
-
 prostaglandin E2 at pH 7.0 Homo sapiens
1.1.1.141 0.0028
-
 prostaglandin E2 at pH 9.0 Homo sapiens
1.1.1.141 0.0048
-
 prostaglandin E1 at pH 9.0 Homo sapiens
1.1.1.141 0.0156
-
 NADH at pH 7.0, + 15-keto-PGE1 Homo sapiens
1.1.1.141 0.0156
-
 NADH at pH 7.0, + 15-ketoprostaglandin E2 Homo sapiens
1.1.1.141 0.0227
-
 NAD+ at pH 9.0, + prostaglandin E2 Homo sapiens
1.1.1.141 0.0266
-
 15-ketoprostaglandin E2 at pH 9.0 Homo sapiens
1.1.1.141 0.0284
-
 NAD+ at pH 7.0, + prostaglandin E2 Homo sapiens
1.1.1.141 0.029
-
 NAD+ at pH 9.0, + prostaglandin E1 Homo sapiens
1.1.1.141 0.0308
-
 NAD+ at pH 7.0, + prostaglandin E1 Homo sapiens
1.1.1.141 0.0521
-
 15-ketoprostaglandin E1 at pH 7.0 Homo sapiens
1.1.1.141 0.0528
-
 15-ketoprostaglandin E2 at pH 7.0 Homo sapiens
1.1.1.141 0.0594
-
 NADH at pH 9.0, + 15-ketoprostaglandin E2 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ Homo sapiens first step in prostaglnadin catabolism (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.141 (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ single displacement mechanism Homo sapiens
a
1.1.1.141 (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ displacement reaction mechanism, at pH 7.0 a kinetically significant ternary complex is formed, while at pH 9.0 the ternary complex is not kinetically significant, Theorell-Chance mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.141 placenta
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.141 -20°C, purified enzyme in a buffer containing 50% glycerol, 5 mM potassium phosphate, pH 7.0, and 1 mM EDTA, loss less than 10% of its initial activity in 2 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ first step in prostaglnadin catabolism Homo sapiens (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ i.e. 15-hydroxyprostaglandin or (15S)-PGE2 Homo sapiens (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
1.1.1.141 prostaglandin E1 + NAD+
-
 Homo sapiens 15-ketoprostaglandin E1 + NADH + H+
-
 ?
1.1.1.141 prostaglandin E2 + NAD+
-
 Homo sapiens 15-ketoprostaglandin E2 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.141 15-hydroxyprostaglandin dehydrogenase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.141 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.141 7 9 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.141 NAD+ specific for Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.141 additional information
-
 additional information inhibition kinetics Homo sapiens