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Literature summary extracted from

  • Novotny, M.J.; Reizer, J.; Esch, F.; Saier, M.H.
    Purification and properties of D-mannitol-1-phosphate dehydrogenase and D-glucitol-6-phosphate dehydrogenase from Escherichia coli (1984), J. Bacteriol., 159, 986-990.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 overexpression in Escherichia coli Escherichia coli
1.1.1.140 cloning and subcloning of the gut operon and gutD gene Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 diethyldicarbonate 60% inhibition at 1 mM Escherichia coli
1.1.1.140 diethyl dicarbonate 100% inactivation Escherichia coli
1.1.1.140 N-ethylmaleimide about 90% inactivation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 0.2
-
 NAD+ cosubstrate: hexitol phosphate Escherichia coli
1.1.1.17 0.8
-
 hexitol phosphate cosubstrate: NAD+ Escherichia coli
1.1.1.140 0.2
-
 NAD+
-
 Escherichia coli
1.1.1.140 3.3
-
 D-sorbitol 6-phosphate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.17 intracellular
-
 Escherichia coli 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 40000
-
 gel filtration Escherichia coli
1.1.1.140 26000
-
 4 * 26000, SDS-PAGE Escherichia coli
1.1.1.140 117000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Escherichia coli highly specific for both substrates D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.17 D-mannitol 1-phosphate + NAD+ Escherichia coli LJ378 highly specific for both substrates D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.17 hexitol phosphate + NAD+ Escherichia coli
-
 ? + NADH
-
 ?
1.1.1.17 hexitol phosphate + NAD+ Escherichia coli LJ378
-
 ? + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Escherichia coli
-
-
-
1.1.1.140 Escherichia coli
-
-
-
1.1.1.140 Escherichia coli LJ378
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 recombinant enzyme Escherichia coli
1.1.1.140
-
 Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 715
-
-
 Escherichia coli
1.1.1.140 180
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.1.140 -20°C, more than 4 months, no appreciable loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ highly specific for both substrates Escherichia coli D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.17 D-mannitol 1-phosphate + NAD+ highly specific for both substrates Escherichia coli LJ378 D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.17 hexitol phosphate + NAD+
-
 Escherichia coli ? + NADH
-
 ?
1.1.1.17 hexitol phosphate + NAD+
-
 Escherichia coli LJ378 ? + NADH
-
 ?
1.1.1.140 D-sorbitol 6-phosphate + NAD+ high specificity for NAD+ Escherichia coli D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.140 D-sorbitol 6-phosphate + NAD+ high specificity for D-sorbitol 6-phosphate Escherichia coli D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.140 D-sorbitol 6-phosphate + NAD+ high specificity for NAD+ Escherichia coli LJ378 D-fructose 6-phosphate + NADH + H+
-
 ?
1.1.1.140 D-sorbitol 6-phosphate + NAD+ high specificity for D-sorbitol 6-phosphate Escherichia coli LJ378 D-fructose 6-phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.17 monomer 1 * 40000, SDS-PAGE Escherichia coli
1.1.1.140 tetramer 4 * 26000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.140 additional information
-
 assay at room temperature Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.17 40
-
 stable during 20 min Escherichia coli
1.1.1.17 60
-
 90% loss of activity after 20 min Escherichia coli
1.1.1.140 40
-
 20 min, 85% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 9
-
 D-mannitol 1-phosphate oxidation Escherichia coli
1.1.1.140 8.7
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+ strictly specific Escherichia coli
1.1.1.140 additional information no activity with NADP+ Escherichia coli
1.1.1.140 NAD+ specific for Escherichia coli