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Literature summary extracted from

  • Schneider, K.H.; Giffhorn, F.
    Sorbitol dehydrogenase from Pseudomonas sp.: Purification, characterization and application to quantitative determination of sorbitol (1991), Enzyme Microb. Technol., 13, 332-337.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.14 dithiothreitol 1 mM 24% stimulation Pseudomonas sp.

General Stability

EC Number General Stability Organism
1.1.1.14 30% sucrose stabilizes Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 CdCl2 1 mM Pseudomonas sp.
1.1.1.14 CoCl2 1 mM Pseudomonas sp.
1.1.1.14 CuSO4 1 mM Pseudomonas sp.
1.1.1.14 DL-2-bromosuccinate 1 mM Pseudomonas sp.
1.1.1.14 FeSO4 1 mM Pseudomonas sp.
1.1.1.14 MgSO4 1 mM Pseudomonas sp.
1.1.1.14 N-ethylmaleimide 1 mM Pseudomonas sp.
1.1.1.14 NiCl2 1 mM Pseudomonas sp.
1.1.1.14 p-chloromercuribenzoate 1 mM Pseudomonas sp.
1.1.1.14 ZnSO4 1 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.09
-
 NADH
-
 Pseudomonas sp.
1.1.1.14 0.24
-
 NAD+
-
 Pseudomonas sp.
1.1.1.14 3.1
-
 D-galactitol
-
 Pseudomonas sp.
1.1.1.14 9.1
-
 D-glucitol
-
 Pseudomonas sp.
1.1.1.14 10
-
 D-tagatose
-
 Pseudomonas sp.
1.1.1.14 176
-
 D-fructose
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 32300
-
 2 * 32300, SDS-PAGE Pseudomonas sp.
1.1.1.14 65800
-
 Stokes' radius, sedimentation coefficient Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 89.5
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-arabinose + NADH low activity Pseudomonas sp. ?
-
 ?
1.1.1.14 D-arabitol + NAD+ D-arabinitol is identical, low activity Pseudomonas sp. ?
-
 ?
1.1.1.14 D-fructose + NADH + H+
-
 Pseudomonas sp. D-sorbitol + NAD+
-
 ?
1.1.1.14 D-galactitol + NAD+
-
 Pseudomonas sp. D-tagatose + NADH
-
 r
1.1.1.14 D-glucitol + NAD+
-
 Pseudomonas sp. D-fructose + NADH + H+
-
 r
1.1.1.14 D-mannitol + NAD+ low activity Pseudomonas sp. ?
-
 ?
1.1.1.14 D-mannose + NADH low activity Pseudomonas sp. ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Pseudomonas sp. D-fructose + NADH + H+
-
 r
1.1.1.14 D-tagatose + NADH
-
 Pseudomonas sp. D-galactitol + NAD+
-
 r
1.1.1.14 D-xylitol + NAD+ low activity Pseudomonas sp. ?
-
 ?
1.1.1.14 D-xylulose + NADH low activity Pseudomonas sp. ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Pseudomonas sp. ?
-
 ?
1.1.1.14 L-sorbose + NADH low activity Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.14 dimer 2 * 32300, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.14 4
-
 50% loss of activity after 4 days Pseudomonas sp.
1.1.1.14 20
-
 50% loss of activity after 32 hours Pseudomonas sp.
1.1.1.14 30
-
 50% loss of activity after 6 hours Pseudomonas sp.
1.1.1.14 40
-
 50% loss of activity after 5 min Pseudomonas sp.
1.1.1.14 50
-
 50% loss of activity after 1 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 6.7
-
 D-fructose reduction Pseudomonas sp.
1.1.1.14 11
-
 sorbitol oxidation Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+
-
 Pseudomonas sp.
1.1.1.14 NADH
-
 Pseudomonas sp.