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Literature summary extracted from

  • Rehg, J.E.; Torack, R.M.
    Partial purification and characterization of sorbitol dehydrogenase from rat brain (1977), J. Neurochem., 28, 655-660.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.14 cysteine fructose reduction at pH 7.4, inhibits at pH 9.0 Rattus norvegicus
1.1.1.14 ZnSO4 3.5 mM reverses EDTA inhibition Rattus norvegicus

General Stability

EC Number General Stability Organism
1.1.1.14 bovine serum albumin stabilizes at 4°C Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 2,3-Dimercaptopropanol
-
 Rattus norvegicus
1.1.1.14 cysteine
-
 Rattus norvegicus
1.1.1.14 EDTA at pH 7.4, not at pH 9.0, fructose reduction Rattus norvegicus
1.1.1.14 iodoacetate
-
 Rattus norvegicus
1.1.1.14 p-hydroxymercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.28
-
 D-sorbitol
-
 Rattus norvegicus
1.1.1.14 0.4
-
 D-xylitol
-
 Rattus norvegicus
1.1.1.14 1.3
-
 D-ribitol
-
 Rattus norvegicus
1.1.1.14 16
-
 D-mannitol
-
 Rattus norvegicus
1.1.1.14 16.7
-
 D-galactitol
-
 Rattus norvegicus
1.1.1.14 20
-
 L-arabitol
-
 Rattus norvegicus
1.1.1.14 54
-
 D-ribulose
-
 Rattus norvegicus
1.1.1.14 77
-
 D-fructose
-
 Rattus norvegicus
1.1.1.14 110
-
 L-sorbose
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.14 sorbitol + NAD+ Rattus norvegicus
-
 D-fructose + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.14 brain
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 -85°C, good preservation Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-fructose + NADH + H+
-
 Rattus norvegicus D-sorbitol + NAD+
-
 ?
1.1.1.14 D-galactitol + NAD+
-
 Rattus norvegicus D-tagatose + NADH
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-ribulose + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Rattus norvegicus D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 L-arabitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 L-sorbose + NADH
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 sorbitol + NAD+
-
 Rattus norvegicus D-fructose + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 7.4 8 D-fructose reduction Rattus norvegicus
1.1.1.14 9 10 sorbitol oxidation Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.14 6 9 at pH 6 about 70% of maximal activity, at pH 9 about 40%, fructose reduction Rattus norvegicus
1.1.1.14 7.5 10 at pH 7.5 about 70% of maximal activity, at pH 10 about 100%, sorbitol oxidation Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+
-
 Rattus norvegicus
1.1.1.14 NADH
-
 Rattus norvegicus
1.1.1.14 NADP+ cannot replace NAD+ Rattus norvegicus
1.1.1.14 NADPH
-
 Rattus norvegicus