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Literature summary extracted from

  • Leissing, N.; McGuinness, E.T.
    Sorbitol dehydrogenase from rat liver (1982), Methods Enzymol., 89, 135-140.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.14 0.1 mg/ml bovine serum albumin stabilizes at 4°C Rattus norvegicus
1.1.1.14 278 mM maltose stabilizes at 4°C Rattus norvegicus
1.1.1.14 6.8 M glycerol stabilizes at 4°C Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 D-fructose above 250 mM, substrate inhibition Rattus norvegicus
1.1.1.14 D-sorbitol above 49 mM, substrate inhibition Rattus norvegicus
1.1.1.14 NAD+ above 7 mM, substrate inhibition Rattus norvegicus
1.1.1.14 NADH above 0.4 mM, substrate inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.067
-
 NADH
-
 Rattus norvegicus
1.1.1.14 0.082
-
 NAD+
-
 Rattus norvegicus
1.1.1.14 0.38
-
 D-sorbitol
-
 Rattus norvegicus
1.1.1.14 136
-
 D-fructose
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 95000
-
 gel filtration Rattus norvegicus
1.1.1.14 97000
-
 disc gel electrophoresis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Ovis aries
-
-
-
1.1.1.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.14 liver
-
 Rattus norvegicus
-
1.1.1.14 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 7.47
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 -20°C, 70% loss of activity after 1 week, 0.1 mg/ml bovine serum albumin stabilizes Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-arabitol + NAD+ D-arabinitol is identical, low activity Rattus norvegicus ?
-
 ?
1.1.1.14 D-arabitol + NAD+ D-arabinitol is identical, low activity Ovis aries ?
-
 ?
1.1.1.14 D-fructose + NADH + H+
-
 Rattus norvegicus D-sorbitol + NAD+
-
 ?
1.1.1.14 D-fructose + NADH + H+
-
 Ovis aries D-sorbitol + NAD+
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Rattus norvegicus D-fructose + NADH + H+
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Ovis aries D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Rattus norvegicus ?
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 L-sorbose + NADH
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.14 tetramer 4 * 35000-40000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.14 22
-
 5 h, stable Rattus norvegicus
1.1.1.14 60
-
 10 min, 65% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 5.9
-
 D-fructose reduction Rattus norvegicus
1.1.1.14 8.1 8.5 sorbitol oxidation Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+
-
 Ovis aries
1.1.1.14 NAD+ specific for Rattus norvegicus
1.1.1.14 NADH
-
 Ovis aries
1.1.1.14 NADH specific for Rattus norvegicus