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Literature summary extracted from

  • Ruffner, H.P.; Rast, D.; Tobler, H.; Karesch, H.
    Purification and properties of mannitol dehydrogenase from Agaricus bisporus sporocarps (1978), Phytochemistry, 17, 865-868.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.138 NADP+ inhibition of fructose reduction, NADP+/NADPH ratio of 4:1 necessary for half maximal activity Agaricus bisporus
1.1.1.138 NADPH inhibition of D-mannitol oxidation, NADP+/NADPH ratio of 0.25 necessary for half maximal activity Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 0.00525
-
 NADPH
-
 Agaricus bisporus
1.1.1.138 34
-
 D-mannitol
-
 Agaricus bisporus
1.1.1.138 190
-
 D-fructose
-
 Agaricus bisporus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.138 Ba2+ 37% activation at 5 mM only in D-mannitol oxidation Agaricus bisporus
1.1.1.138 Ca2+ 52% activation at 5 mM up to 140% at 10 mM only in D-mannitol oxidation Agaricus bisporus
1.1.1.138 Mg2+ 36% activation at 5 mM only in D-mannitol oxidation Agaricus bisporus
1.1.1.138 Mn2+ 15% activation at 5 mM only in D-mannitol oxidation Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 40000
-
 gel filtration Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-fructose + NADPH Agaricus bisporus
-
 D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NADP+ Agaricus bisporus
-
 D-fructose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Agaricus bisporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138 to homogeneity, affinity chromatography Agaricus bisporus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.138 sporocarp
-
 Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 148.6
-
-
 Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-arabinitol + NADP+ 3% of the activity compared to D-mannitol Agaricus bisporus D-xylulose + NADPH + H+
-
 ?
1.1.1.138 D-fructose + NADPH
-
 Agaricus bisporus D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Agaricus bisporus D-fructose + NADPH + H+
-
 r
1.1.1.138 D-sorbitol + NADP+ 21% of the activity compared to D-mannitol Agaricus bisporus D-sorbose + NADPH + H+
-
 ?
1.1.1.138 L-arabinitol + NADP+ 2% of the activity compared to D-mannitol Agaricus bisporus L-xylulose + NADPH + H+
-
 ?
1.1.1.138 perseitol + NADP+ 6% of the activity compared to D-mannitol Agaricus bisporus ? + NADPH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.138 47
-
-
 Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 7.9 8.1 D-fructose reduction Agaricus bisporus
1.1.1.138 8.7 8.8 D-mannitol oxidation Agaricus bisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+ specific for NADP+ Agaricus bisporus
1.1.1.138 NADPH cannot be replaced by NADH Agaricus bisporus