BRENDA - Enzyme Database

Polygalacturonic acid metabolism in bacteria. II. Formation and metabolism of 3-deoxy-D-glycero-2,5-hexodiulosonic acid

Preiss, J.; Ashwell, G.; J. Biol. Chem. 238, 1577-1583 (1963)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.127
1 mM NAD+ or NADP+ increase stability
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
Pseudomonas sp.
irreversible at pH 7.0
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
Pseudomonas sp.
ir
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.127
Pseudomonas sp.
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.127
partial
Pseudomonas sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.127
cell culture
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.127
19.5
-
-
Pseudomonas sp.
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.127
0-3░C, 1 mM NAD+ or NADP+, 50-67% retaining activity after 5 weeks
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
irreversible at pH 7.0
285821
Pseudomonas sp.
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
285821
Pseudomonas sp.
ir
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.127
7
8
-
Pseudomonas sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.127
NAD+
-
Pseudomonas sp.
1.1.1.127
NADP+
-
Pseudomonas sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.127
NAD+
-
Pseudomonas sp.
1.1.1.127
NADP+
-
Pseudomonas sp.
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.127
1 mM NAD+ or NADP+ increase stability
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
Pseudomonas sp.
irreversible at pH 7.0
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
Pseudomonas sp.
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.127
partial
Pseudomonas sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.127
cell culture
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.127
19.5
-
-
Pseudomonas sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.127
0-3░C, 1 mM NAD+ or NADP+, 50-67% retaining activity after 5 weeks
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
irreversible at pH 7.0
285821
Pseudomonas sp.
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
285821
Pseudomonas sp.
ir
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.127
7
8
-
Pseudomonas sp.