BRENDA - Enzyme Database

An enzyme in the pectinolytic pathway of Erwinia chrysanthemi: 2-Keto-3-deoxygluconate oxidoreductase

Condemine, G.; Hugouvieux-Cotte-Pattat, N.; Robert-Baudouy, J.; J. Gen. Microbiol. 130, 2839-2844 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.127
Ag+
72% inhibition at 0.01 mM
Dickeya chrysanthemi
1.1.1.127
ATP
30% inhibition for both reduction and oxidation reaction at 1 mM
Dickeya chrysanthemi
1.1.1.127
p-chloromercuribenzoate
50% inhibition at 0.02 mM
Dickeya chrysanthemi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.127
0.03
-
NADH
-
Dickeya chrysanthemi
1.1.1.127
0.34
-
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
-
Dickeya chrysanthemi
1.1.1.127
0.4
-
NAD+
-
Dickeya chrysanthemi
1.1.1.127
7.7
-
2-dehydro-3-deoxy-D-gluconate
-
Dickeya chrysanthemi
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.127
Ca2+
20% activation at 1 mM
Dickeya chrysanthemi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
Dickeya chrysanthemi
involved in pectinolysis by some phytopathogenic bacteria
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
Dickeya chrysanthemi
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.127
Dickeya chrysanthemi
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.127
to homogeneity, 2step chromatography
Dickeya chrysanthemi
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.127
cell culture
-
Dickeya chrysanthemi
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.127
39
-
-
Dickeya chrysanthemi
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.127
-20°C, 2 mM DTT, 6 months
Dickeya chrysanthemi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
involved in pectinolysis by some phytopathogenic bacteria
285820
Dickeya chrysanthemi
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
285820
Dickeya chrysanthemi
r
1.1.1.127
pyruvate + NAD+
10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate
285820
Dickeya chrysanthemi
? + NADH
-
285820
Dickeya chrysanthemi
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.127
45
-
-
Dickeya chrysanthemi
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.127
20
-
stable for 15 h
Dickeya chrysanthemi
1.1.1.127
46
-
half-life: 10 min, NADH increases thermal stability
Dickeya chrysanthemi
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.127
7
7.5
reduction reaction
Dickeya chrysanthemi
1.1.1.127
10
-
oxidation reaction
Dickeya chrysanthemi
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.127
NAD+
-
Dickeya chrysanthemi
1.1.1.127
NADH
-
Dickeya chrysanthemi
1.1.1.127
NADP+
53% lower activity than with NAD+
Dickeya chrysanthemi
1.1.1.127
NADPH
62% lower activity than with NADH
Dickeya chrysanthemi
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.127
NAD+
-
Dickeya chrysanthemi
1.1.1.127
NADH
-
Dickeya chrysanthemi
1.1.1.127
NADP+
53% lower activity than with NAD+
Dickeya chrysanthemi
1.1.1.127
NADPH
62% lower activity than with NADH
Dickeya chrysanthemi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.127
Ag+
72% inhibition at 0.01 mM
Dickeya chrysanthemi
1.1.1.127
ATP
30% inhibition for both reduction and oxidation reaction at 1 mM
Dickeya chrysanthemi
1.1.1.127
p-chloromercuribenzoate
50% inhibition at 0.02 mM
Dickeya chrysanthemi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.127
0.03
-
NADH
-
Dickeya chrysanthemi
1.1.1.127
0.34
-
(4S)-4,6-dihydroxy-2,5-dioxohexanoate
-
Dickeya chrysanthemi
1.1.1.127
0.4
-
NAD+
-
Dickeya chrysanthemi
1.1.1.127
7.7
-
2-dehydro-3-deoxy-D-gluconate
-
Dickeya chrysanthemi
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.127
Ca2+
20% activation at 1 mM
Dickeya chrysanthemi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
Dickeya chrysanthemi
involved in pectinolysis by some phytopathogenic bacteria
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
Dickeya chrysanthemi
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.127
to homogeneity, 2step chromatography
Dickeya chrysanthemi
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.127
cell culture
-
Dickeya chrysanthemi
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.127
39
-
-
Dickeya chrysanthemi
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.127
-20°C, 2 mM DTT, 6 months
Dickeya chrysanthemi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.127
2-dehydro-3-deoxy-D-gluconate + NAD+
involved in pectinolysis by some phytopathogenic bacteria
285820
Dickeya chrysanthemi
(4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
285820
Dickeya chrysanthemi
r
1.1.1.127
pyruvate + NAD+
10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate
285820
Dickeya chrysanthemi
? + NADH
-
285820
Dickeya chrysanthemi
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.127
45
-
-
Dickeya chrysanthemi
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.127
20
-
stable for 15 h
Dickeya chrysanthemi
1.1.1.127
46
-
half-life: 10 min, NADH increases thermal stability
Dickeya chrysanthemi
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.127
7
7.5
reduction reaction
Dickeya chrysanthemi
1.1.1.127
10
-
oxidation reaction
Dickeya chrysanthemi