EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.127 | Ag+ | 72% inhibition at 0.01 mM | Dickeya chrysanthemi | |
1.1.1.127 | ATP | 30% inhibition for both reduction and oxidation reaction at 1 mM | Dickeya chrysanthemi | |
1.1.1.127 | p-chloromercuribenzoate | 50% inhibition at 0.02 mM | Dickeya chrysanthemi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.127 | 0.03 | - |
NADH | - |
Dickeya chrysanthemi | |
1.1.1.127 | 0.34 | - |
(4S)-4,6-dihydroxy-2,5-dioxohexanoate | - |
Dickeya chrysanthemi | |
1.1.1.127 | 0.4 | - |
NAD+ | - |
Dickeya chrysanthemi | |
1.1.1.127 | 7.7 | - |
2-dehydro-3-deoxy-D-gluconate | - |
Dickeya chrysanthemi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.127 | Ca2+ | 20% activation at 1 mM | Dickeya chrysanthemi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.127 | 2-dehydro-3-deoxy-D-gluconate + NAD+ | Dickeya chrysanthemi | involved in pectinolysis by some phytopathogenic bacteria | (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.127 | Dickeya chrysanthemi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.127 | to homogeneity, 2step chromatography | Dickeya chrysanthemi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.127 | cell culture | - |
Dickeya chrysanthemi | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 39 | - |
- |
Dickeya chrysanthemi |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.127 | -20°C, 2 mM DTT, 6 months | Dickeya chrysanthemi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.127 | 2-dehydro-3-deoxy-D-gluconate + NAD+ | involved in pectinolysis by some phytopathogenic bacteria | Dickeya chrysanthemi | (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH | - |
r | |
1.1.1.127 | pyruvate + NAD+ | 10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate | Dickeya chrysanthemi | ? + NADH | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 45 | - |
- |
Dickeya chrysanthemi |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 20 | - |
stable for 15 h | Dickeya chrysanthemi |
1.1.1.127 | 46 | - |
half-life: 10 min, NADH increases thermal stability | Dickeya chrysanthemi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.127 | 7 | 7.5 | reduction reaction | Dickeya chrysanthemi |
1.1.1.127 | 10 | - |
oxidation reaction | Dickeya chrysanthemi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.127 | NAD+ | - |
Dickeya chrysanthemi | |
1.1.1.127 | NADH | - |
Dickeya chrysanthemi | |
1.1.1.127 | NADP+ | 53% lower activity than with NAD+ | Dickeya chrysanthemi | |
1.1.1.127 | NADPH | 62% lower activity than with NADH | Dickeya chrysanthemi |