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Literature summary extracted from

  • Ameyama, M.; Matsushita, K.; Shinagawa, E.; Adachi, O.
    5-keto-D-Fructose reductase of Gluconobacter industrius: Purification, crystallization and properties (1981), Agric. Biol. Chem., 45, 863-869.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.124 ammonium sulfate precipitation Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.124 sulfhydryl reagents
-
 Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.124 0.008
-
 NADPH
-
 Gluconobacter oxydans
1.1.1.124 4.5
-
 5-keto-D-fructose
-
 Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.124 25000
-
 4 * 25000, SDS-PAGE Gluconobacter oxydans
1.1.1.124 100000
-
 gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.124 D-fructose + NADP+ Gluconobacter oxydans predominant reduction of 5-keto-D-fructose 5-dehydro-D-fructose + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.124 Gluconobacter oxydans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.124 high purity to crystalline form, chromatography techniques Gluconobacter oxydans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.124 cell culture
-
 Gluconobacter oxydans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.124 3 5 D-fructose oxidation Gluconobacter oxydans
1.1.1.124 620
-
 5-keto-D-fructose reduction Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.124 D-fructose + NADP+ predominant reduction of 5-keto-D-fructose Gluconobacter oxydans 5-dehydro-D-fructose + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.124 tetramer 4 * 25000, SDS-PAGE Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.124 6.5
-
 5-keto-D-fructose reduction Gluconobacter oxydans
1.1.1.124 8.5 10 D-fructose oxidation Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.124 4 8 stable between Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.124 NADPH highly specific Gluconobacter oxydans