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Literature summary extracted from

  • Schrimsher, J.L.; Wingfield, P.T.; Bernard, A.; Mattaliano, R.; Payton, M.A.
    Purification and characterization of 5-ketofructose reductase from Erwinia citreus (1988), Biochem. J., 253, 511-516.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.124 D-fructose non-competitive inhibition Erwinia sp.
1.1.1.124 NADP+ competitive inhibition of D-fructose synthesis, non-competitive inhibition of 5-keto-fructose synthesis Erwinia sp.
1.1.1.124 NADPH competitive inhibition of D-fructose oxidation Erwinia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.124 0.053
-
 NADP+
-
 Erwinia sp.
1.1.1.124 0.071
-
 NADPH
-
 Erwinia sp.
1.1.1.124 0.32
-
 NADH
-
 Erwinia sp.
1.1.1.124 1.97
-
 NAD+
-
 Erwinia sp.
1.1.1.124 9.1
-
 D-fructose
-
 Erwinia sp.
1.1.1.124 12.7
-
 5-keto-D-fructose
-
 Erwinia sp.
1.1.1.124 140
-
 D-arabinose
-
 Erwinia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.124 40000
-
 1 * 40000, SDS-PAGE Erwinia sp.
1.1.1.124 50000
-
 gel filtration, sedimentation equilibrium measurement Erwinia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.124 D-fructose + NADP+ Erwinia sp. highly specific for D-fructose 5-dehydro-D-fructose + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.124 Erwinia sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.124 to homogeneity, chromatography techniques Erwinia sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.124 cell culture
-
 Erwinia sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.124 2450
-
-
 Erwinia sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.124 -20°C, 30 mM Tris-HCl, pH 7.8, 3 months Erwinia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.124 5-dehydro-D-fructose + NADH + H+
-
 Erwinia sp. D-fructose + NAD+
-
 r
1.1.1.124 D-arabinose + NADP+ 5fold lower activity than for D-fructose Erwinia sp. ? + NADPH
-
 ?
1.1.1.124 D-fructose + NAD+
-
 Erwinia sp. 5-dehydro-D-fructose + NADH + H+
-
 r
1.1.1.124 D-fructose + NADP+ highly specific for D-fructose Erwinia sp. 5-dehydro-D-fructose + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.124 monomer 1 * 40000, SDS-PAGE Erwinia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.124 7
-
 reduction of 5-keto-D-fructose Erwinia sp.
1.1.1.124 9.5
-
 oxidation of D-fructose Erwinia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.124 NADH substitutes for NADPH Erwinia sp.
1.1.1.124 NADPH oxidized and reduced cofactors bind to the same site of the enzyme Erwinia sp.