Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Englard, S.; Avigad, G.
    5-keto-D-Fructose reductase from yeast (1975), Methods Enzymol., 41B, 132-138.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.123 NADP+ competitive inhibitor Saccharomyces cerevisiae
1.1.1.123 p-hydroxymercuribenzoate 0.4 mM and 1 mM inhibit activity to 21% and 41% respectively Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.123 0.046
-
 NADPH pH 7.5, 30°C Saccharomyces cerevisiae
1.1.1.123 0.29
-
 5-Keto-D-fructose 1-phosphate
-
 Saccharomyces cerevisiae
1.1.1.123 1
-
 5-keto-D-fructose pH 7.5, 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.123 Bacillus subtilis
-
 var. aterneum
-
1.1.1.123 Bacillus subtilis
-
 168-2
-
1.1.1.123 Bacillus subtilis 168-2
-
 168-2
-
1.1.1.123 Brevibacillus brevis
-
 9999
-
1.1.1.123 Cyberlindnera jadinii
-
-
-
1.1.1.123 Enterobacter cloacae
-
-
-
1.1.1.123 Gluconobacter oxydans
-
 Acetobacter melanogenus
-
1.1.1.123 Klebsiella aerogenes
-
-
-
1.1.1.123 no activity in Candida tropicalis
-
-
-
1.1.1.123 Priestia megaterium
-
-
-
1.1.1.123 Proteus vulgaris
-
-
-
1.1.1.123 Saccharomyces cerevisiae
-
 Boehringer, baker's and brewer's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.123 ammonium sulfate precipitation, affinity chromatography, ion exchange chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.123 28.4
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.1.123 4°C, purified preparations, stable for at least 4 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.123 5-keto-D-fructose + NADPH
-
 Saccharomyces cerevisiae L-sorbose + NADP+
-
 ir
1.1.1.123 5-keto-D-fructose 1-phosphate + NADPH low activity, maximal rate of 1.8% that observed for 5-keto-D-fructose Saccharomyces cerevisiae L-sorbose 1-phosphate + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.123 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.123 7.4
-
 assay at Saccharomyces cerevisiae
1.1.1.123 7.4 8.5 Tris-acetate buffer Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.123 NADH
-
 Gluconobacter oxydans
1.1.1.123 NADPH
-
 Saccharomyces cerevisiae