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Literature summary extracted from

  • Guimaraes, M.F.; Veiga, L.A.
    Purification and general properties of L-fucose dehydrogenase from Pullularia pullulans (1989), Arq. Biol. Tecnol. (Curitiba), 32, 575-587.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.122 heavy metal ions strong inhibition Aureobasidium pullulans
1.1.1.122 thiol reagents strong inhibition Aureobasidium pullulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.122 0.33
-
 NAD+
-
 Aureobasidium pullulans
1.1.1.122 1.96
-
 6-deoxy-L-galactose
-
 Aureobasidium pullulans
1.1.1.122 40
-
 D-arabinose
-
 Aureobasidium pullulans
1.1.1.122 50
-
 L-galactose
-
 Aureobasidium pullulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.122 40000
-
 gel filtration Aureobasidium pullulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.122 6-deoxy-L-galactose + NAD+ Aureobasidium pullulans i.e. L-fucose 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
1.1.1.122 D-arabinose + NAD+ Aureobasidium pullulans
-
 D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.122 L-galactose + NAD+ Aureobasidium pullulans
-
 L-galactono-1,5-lactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.122 Aureobasidium pullulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.122 to homogeneity, chromatography techniques Aureobasidium pullulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.122 cell culture inducible Aureobasidium pullulans
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.122 -20°C, 2 months Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.122 6-deoxy-L-galactose + NAD+ i.e. L-fucose Aureobasidium pullulans 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
1.1.1.122 D-arabinose + NAD+
-
 Aureobasidium pullulans D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.122 L-galactose + NAD+
-
 Aureobasidium pullulans L-galactono-1,5-lactone + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.122 monomer 1 * 40000, SDS-PAGE Aureobasidium pullulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.122 4
-
 rapid loss of activity Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.122 10.5
-
-
 Aureobasidium pullulans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.122 NAD+ NAD-specific Aureobasidium pullulans