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Literature summary extracted from

  • Cline, A.L.; Hu, A.S.L.
    Enzymatic characterization and comparison of three sugar dehydrogenases from a pseudomonad (1965), J. Biol. Chem., 240, 4493-4497.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.117 0.82
-
 D-arabinose
-
 Pseudomonas sp.
1.1.1.117 2.3
-
 NADP+
-
 Pseudomonas sp.
1.1.1.117 22
-
 NAD+
-
 Pseudomonas sp.
1.1.1.121 0.16
-
 NAD+
-
 Pseudomonas sp.
1.1.1.121 0.78
-
 D-galactose
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.117 cytoplasm
-
 Pseudomonas sp. 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.117 D-arabinose + NAD(P)+ Pseudomonas sp. specific for sugars of the optical configuration of L-galactose D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.120 alpha-D-galactose + NADP+ Pseudomonas sp. 39% of the activity compared to alpha,beta-D-galactose and alpha,beta-L-arabinose D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 beta-D-galactose + NADP+ Pseudomonas sp.
-
 D-galatonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 D-galactose + NADP+ Pseudomonas sp.
-
 D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.121 D-aldose + NAD+ Pseudomonas sp. broad substrate specificity D-aldonolactone + NADH
-
 ?
1.1.1.121 D-aldose + NAD+ Pseudomonas sp. the enzyme is coordinately regulated with EC 1.1.1.120 and EC 1.1.1.117 D-aldonolactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.117 Pseudomonas sp.
-
 G6
-
1.1.1.117 Pseudomonas sp. G6
-
 G6
-
1.1.1.120 Pseudomonas sp.
-
 mutant strain G6
-
1.1.1.121 Pseudomonas sp.
-
 G6
-
1.1.1.121 Pseudomonas sp. G6
-
 G6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.117
-
 Pseudomonas sp.
1.1.1.121 G6 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.120 cell culture
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.117 D-arabinose + NAD(P)+ alpha-anomer preferred Pseudomonas sp. D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.117 D-arabinose + NAD(P)+ specific for sugars of the optical configuration of L-galactose Pseudomonas sp. D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.117 D-arabinose + NAD+
-
 Pseudomonas sp. D-arabinono-1,4-lactone + NADH + H+
-
 r
1.1.1.117 L-colitose + NAD(P)+ i.e. 3,6-dideoxy-L-galactose Pseudomonas sp. 3,6-dideoxy-L-galactono-1,5-lactone + NAD(P)H
-
 ?
1.1.1.117 L-fucose + NADP+ i.e. 6-deoxy-L-galactose Pseudomonas sp. 6-deoxy-L-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.117 L-galactose + NADP+ beta-anomer preferred Pseudomonas sp. L-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.120 2-deoxy-D-galactose + NADP+ 90% of the activity compared to D-galactose Pseudomonas sp. D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 2-deoxy-D-galactose + NADP+ 90% of the activity compared to D-galactose Pseudomonas sp. G6 D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 6-deoxy-D-galactose + NADP+ 156% of the activity compared to D-galactose Pseudomonas sp. D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 6-deoxy-D-galactose + NADP+ 156% of the activity compared to D-galactose Pseudomonas sp. G6 D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 alpha,beta-L-arabinose + NADP+
-
 Pseudomonas sp. L-arabinolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 alpha,beta-L-arabinose + NADP+
-
 Pseudomonas sp. G6 L-arabinolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 alpha-D-galactose + NADP+ 39% of the activity compared to alpha,beta-D-galactose and alpha,beta-L-arabinose Pseudomonas sp. D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 beta-D-galactofuranose + NADP+
-
 Pseudomonas sp. D-galactono-1,4-lactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 beta-D-galactofuranose + NADP+
-
 Pseudomonas sp. G6 D-galactono-1,4-lactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 beta-D-galactose + NADP+
-
 Pseudomonas sp. D-galatonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 beta-L-arabinose + NADP+ 63% of the activity compared to alpha,beta-L-arabinose and alpha,beta-D-galactose Pseudomonas sp. L-arabinolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 beta-L-arabinose + NADP+ 63% of the activity compared to alpha,beta-L-arabinose and alpha,beta-D-galactose Pseudomonas sp. G6 L-arabinolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 D-galactose + NADP+
-
 Pseudomonas sp. D-galactonolactone + NADPH product is assumed to be the corresponding -1,5-lactone ?
1.1.1.120 L-arabinose + NADP+ 44% of the activity compared to D-galactose Pseudomonas sp. L-arabino-1,5-lactone + NADPH + H+ product is assumed to be the corresponding -1,5-lactone ?
1.1.1.121 2,3,4-trideoxyaldopyranose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 2-deoxy-D-galactose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 2-deoxy-D-glucose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 5-hydroxypentanal + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 chalcose + NAD+ demethylated Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 D-aldose + NAD+ broad substrate specificity Pseudomonas sp. D-aldonolactone + NADH
-
 ?
1.1.1.121 D-aldose + NAD+ the enzyme is coordinately regulated with EC 1.1.1.120 and EC 1.1.1.117 Pseudomonas sp. D-aldonolactone + NADH
-
 ?
1.1.1.121 D-fucose + NAD+
-
 Pseudomonas sp. D-fucono-delta-lactone + NADH
-
 ?
1.1.1.121 D-galactose + NAD+ beta-anomer preferred to alpha-anomer Pseudomonas sp. D-galactono-gamma-lactone + NADH
-
 ?
1.1.1.121 D-glucose + NAD+ the most probable produced aldonolactones from this and the following substrates have not been isolated so far Pseudomonas sp. D-glucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-glucose + NAD+ beta-anomer preferred over alpha-anomer Pseudomonas sp. D-glucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-quinovose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 D-xylose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 L-arabinose + NAD+ alpha-anomer preferred to beta-anomer Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 additional information broad substrate specificity Pseudomonas sp. ?
-
 ?
1.1.1.121 viosamine + NAD+ i.e. 4-amino-4,6-dideoxy-D-glucose Pseudomonas sp. ? + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.117 23
-
 assay at Pseudomonas sp.
1.1.1.121 23
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.117 8 8.5
-
 Pseudomonas sp.
1.1.1.121 9.2
-
 Tris/acetate buffer Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.117 7.3 9.2 about 80% of maximal activity at pH 7.3 and 9.2 Pseudomonas sp.
1.1.1.121 8 9.8 about 80% of maximal activity at pH 8.0 and 9.8 in Tris/acetate buffer Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.117 NAD+ the affinity for NADP+ is considerably higher Pseudomonas sp.
1.1.1.117 NADP+
-
 Pseudomonas sp.
1.1.1.120 NADP+ cannot be replaced by NAD+ Pseudomonas sp.
1.1.1.121 NAD+
-
 Pseudomonas sp.
1.1.1.121 NADP+ active at concentrations above 0.02 M Pseudomonas sp.