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Literature summary extracted from

  • Kim, S.T.; Huh, W.K.; Lee, B.H.; Kang, S.O.
    D-Arabinose dehydrogenase and its gene from Saccharomyces cerevisiae (1998), Biochim. Biophys. Acta, 1429, 29-39.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.427 24
-
 L-Xylose
-
 Saccharomyces cerevisiae
1.1.1.427 98
-
 L-fucose
-
 Saccharomyces cerevisiae
1.1.1.427 161
-
 D-arabinose
-
 Saccharomyces cerevisiae
1.1.1.427 180
-
 L-galactose
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.427 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.427 39000
-
 1 * 39000 + 1 * 40000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.427 40000
-
 1 * 39000 + 1 * 40000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.427 40000
-
 1 * 40000 + 1 + 39000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.427 74000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.427 D-arabinose + NAD(P)+ Saccharomyces cerevisiae
-
 D-arabino-1,4-lactone + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.427 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.427
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.427 101
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.427 D-arabinose + NAD(P)+
-
 Saccharomyces cerevisiae D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.427 D-arabinose + NAD+
-
 Saccharomyces cerevisiae D-arabinono-1,4-lactone + NADH
-
 ir
1.1.1.427 D-arabinose + NADP+
-
 Saccharomyces cerevisiae D-arabinono-1,4-lactone + NADPH
-
 ?
1.1.1.427 D-arabinose + NADP+
-
 Saccharomyces cerevisiae D-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.427 D-xylose + NADP+
-
 Saccharomyces cerevisiae D-xylonolactone + NADPH + H+
-
 ?
1.1.1.427 L-fucose + NADP+
-
 Saccharomyces cerevisiae 6-deoxy-L-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.427 L-fucose + NADP+ with NADP+ Saccharomyces cerevisiae L-fuconolactone + NADPH + H+
-
 ?
1.1.1.427 L-galactose + NADP+
-
 Saccharomyces cerevisiae L-galactono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.427 L-xylose + NADP+
-
 Saccharomyces cerevisiae L-xylono-1,4-lactone + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.427 dimer 1 * 39000 + 1 * 40000, SDS-PAGE Saccharomyces cerevisiae
1.1.1.427 heterodimer 1 * 40000 + 1 + 39000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.427 30
-
-
 Saccharomyces cerevisiae
1.1.1.427 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.427 10
-
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.427 NADP+
-
 Saccharomyces cerevisiae