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Literature summary extracted from
- Indolelactate dehydrogenase from Clostridium sporogenes (1968), Can. J. Microbiol., 14, 429-435.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.110 | 0.0142 | - |
NADH | - |
Clostridium sporogenes |  |
| 1.1.1.110 | 0.075 | - |
p-hydroxyphenylpyruvate | - |
Clostridium sporogenes | |
| 1.1.1.110 | 0.106 | - |
phenylpyruvate | - |
Clostridium sporogenes | |
| 1.1.1.110 | 0.195 | - |
NAD+ | - |
Clostridium sporogenes | |
| 1.1.1.110 | 0.691 | - |
indoleglycolate | - |
Clostridium sporogenes | |
| 1.1.1.110 | 1.03 | - |
indolelactate | - |
Clostridium sporogenes | |
| 1.1.1.110 | 1.77 | - |
p-hydroxyphenyllactate | - |
Clostridium sporogenes | |
| 1.1.1.110 | 2.21 | - |
Phenyllactate | - |
Clostridium sporogenes | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.110 | indolelactate + NAD+ | Clostridium sporogenes | - |
indolepyruvate + NADH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.110 | Clostridium sporogenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.110 | ammonium sulfate, DEAE-cellulose | Clostridium sporogenes |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.110 | -20°C, 100 mM potassium phosphate, pH 7.5, 1 mM mercaptoethanol, 6 months, no loss of activity, 0°C, 100 mM potassium phosphate, pH 7.5, 1 mM mercaptoethanol, 15 days, no loss of activity | Clostridium sporogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.110 | indoleglycolate + NAD+ | - |
Clostridium sporogenes | indoleglyoxylate + NADH | - |
r | |
| 1.1.1.110 | indolelactate + NAD+ | - |
Clostridium sporogenes | indolepyruvate + NADH | - |
r | |
| 1.1.1.110 | p-hydroxyphenyllactate + NAD+ | - |
Clostridium sporogenes | p-hydroxyphenylpyruvate + NADH | - |
r | |
| 1.1.1.110 | phenyllactate + NAD+ | - |
Clostridium sporogenes | phenylpyruvate + NADH | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.110 | 6 | 8 | indolepyruvate reduction | Clostridium sporogenes |
| 1.1.1.110 | 8 | 9 | indolelactate oxidation | Clostridium sporogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.110 | NAD+ | - |
Clostridium sporogenes | |
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Release 2023.1 (January 2023)
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