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Literature summary extracted from

  • Hallborn, J.; Walfridsson, M.; Penttilae, M.; Keraenen, S.; Hahn-Haegerdal, B.
    A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity (1995), Yeast, 11, 839-847.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.11 gene over-expressed in Saccharomyces cerevisiae S700 Scheffersomyces stipitis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.11 0.2
-
 NAD+
-
 Scheffersomyces stipitis
1.1.1.11 20
-
 D-arabinitol
-
 Scheffersomyces stipitis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.11 additional information no divalent cations are required for activity Scheffersomyces stipitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.11 30000
-
 4 * 30000, calculated from gene sequence and molecular weight of native enzyme Scheffersomyces stipitis
1.1.1.11 110000
-
 native gradient PAGE Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.11 D-arabinitol + NAD+ Scheffersomyces stipitis recombinant protein from Saccharomyces cerevisiae D-ribulose + NADH + H+
-
 ?
1.1.1.11 D-arabinitol + NAD+ Scheffersomyces stipitis D-arabitol i.e. D-arabinitol D-xylulose + NADH + H+
-
 ?
1.1.1.11 additional information Scheffersomyces stipitis D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.11 Scheffersomyces stipitis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.11 0.2
-
 D-xylulose, of recombinant protein Scheffersomyces stipitis
1.1.1.11 0.78
-
-
 Scheffersomyces stipitis
1.1.1.11 68.25
-
 D-arabinitol, of recombinant protein Scheffersomyces stipitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.11 D-arabinitol + NAD+ recombinant protein from Saccharomyces cerevisiae Scheffersomyces stipitis D-ribulose + NADH + H+
-
 ?
1.1.1.11 D-arabinitol + NAD+ D-arabitol i.e. D-arabinitol Scheffersomyces stipitis D-xylulose + NADH + H+
-
 ?
1.1.1.11 D-glucitol + NAD+ recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis Scheffersomyces stipitis ?
-
 ?
1.1.1.11 Galactitol + NAD+ recombinant protein from Saccharomyces cerevisiae Scheffersomyces stipitis ?
-
 ?
1.1.1.11 additional information D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose Scheffersomyces stipitis ?
-
 ?
1.1.1.11 Xylitol + NAD+ recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis Scheffersomyces stipitis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.11 tetramer 4 * 30000, calculated from gene sequence and molecular weight of native enzyme Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.11 additional information no activity with NADPH Scheffersomyces stipitis