Literature summary extracted from

McGilvray, D.; Morris, J.G.
L-Threonine dehydrogenase (Arthrobacter) (1971), Methods Enzymol., 17B, 580-584.

No PubMed abstract available

General Stability

EC Number	General Stability	Organism
1.1.1.103	most stable in Tris-HCl, pH 8, purified preparations in phosphate buffers lose their activity overnight	Arthrobacter sp.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.103	Ag+	-	Arthrobacter sp.
1.1.1.103	EDTA	-	Arthrobacter sp.
1.1.1.103	Hg2+	-	Arthrobacter sp.
1.1.1.103	p-mercuribenzoate	-	Arthrobacter sp.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.103	0.14	-	NAD+	at pH 7.5	Arthrobacter sp.
1.1.1.103	10.5	-	L-threonine	at pH 7.5	Arthrobacter sp.

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.1.103	Co2+	restores enzyme activity after EDTA treatment	Arthrobacter sp.
1.1.1.103	Mn2+	restores enzyme activity after EDTA treatment	Arthrobacter sp.
1.1.1.103	Zn2+	restores enzyme activity after EDTA treatment	Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.103	L-threonine + NAD+	Arthrobacter sp.	-	(2S)-2-amino-3-oxobutanoate + NADH + H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.103	Arthrobacter sp.	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.103	protamine sulfate, Sephadex G-200, DEAE-cellulose	Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.103	6.05	-	-	Arthrobacter sp.

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.103	L-threonine + NAD+	-	Arthrobacter sp.	(2S)-2-amino-3-oxobutanoate + NADH + H+	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.103	8.5	8.9	-	Arthrobacter sp.

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.103	NAD+	-	Arthrobacter sp.

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Release 2023.1 (January 2023)