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Literature summary extracted from

  • Aoyama, Y.; Motokawa, Y.
    L-Threonine dehydrogenase of chicken liver (1981), J. Biol. Chem., 256, 12367-12373.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.103 adenosine-5'-diphosphoribose competive inhibition vs. NAD+, noncompetitive inhibition vs. L-threonine Gallus gallus
1.1.1.103 Cd2+ 0.05 and 1.0 mM, 90% inhibition Gallus gallus
1.1.1.103 Cu2+ 0.05 and 1.0 mM, 90% inhibition Gallus gallus
1.1.1.103 Mn2+ 1.0 mM, 40-50% inhibition Gallus gallus
1.1.1.103 NADH competitive inhibition vs. NAD+, noncompetitive vs. L-threonine Gallus gallus
1.1.1.103 Ni2+ 1 mM, 40-50% inhibition Gallus gallus
1.1.1.103 Zn2+ 0.05 and 1.0 mM, 90% inhibition Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 0.98
-
NAD+
-
Gallus gallus
1.1.1.103 8.4
-
L-threonine
-
Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 78000
-
gel filtration Gallus gallus
1.1.1.103 88000
-
density gradient Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.103 L-threonine + NAD+ Gallus gallus
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 DEAE-cellulose, ammonium sulfate, DEAE-cellulose, Blue dextran-Sepharose, Sephadex G-200, amylamine sepharose, Sephadex G-200 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.103 liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.103 19
-
-
Gallus gallus

Storage Stability

EC Number Storage Stability Organism
1.1.1.103 -20°C, 14 mM 2-mercaptoethanol, 20% glycerol, at least 1 month, little loss of activity Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-threonine + NAD+
-
Gallus gallus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 monomer 1 * 88000, SDS-PAGE Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 8.6 8.7 30% activity at pH 7.5, 80% activity at pH 9.8 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+
-
Gallus gallus