EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.103 | iodoacetamide | 3.2 mM, 10% inhibition | Escherichia coli K-12 | |
1.1.1.103 | iodoacetate | 15% protection against inhibition with 5 mM NAD+, 30% with 5 mM L-threonine, 60-70% protection in the presence of both NAD+ and L-threonine, inactivation occurs more rapidly in the presence of Cd2+; enzyme contains 6 half-cystine residues per subunit, 2 disulfide bonds and 4 sulfhydryl groups | Escherichia coli K-12 | |
1.1.1.103 | methylmethanethiosulfonate | 0.4 mM, 350fold molar excess over enzyme sulfhydryl groups leads to complete inactivation | Escherichia coli K-12 | |
1.1.1.103 | p-mercuribenzoate | 10fold excess leads to immidiate inactivation | Escherichia coli K-12 | |
1.1.1.103 | thionitrobenzoate | 40fold molar excess, gradual 99% loss of enzyme activity | Escherichia coli K-12 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.103 | Cd2+ | activation is not thiol-dependent | Escherichia coli K-12 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.103 | L-threonine + NAD+ | Escherichia coli K-12 | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.103 | Escherichia coli K-12 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.103 | L-threonine + NAD+ | - |
Escherichia coli K-12 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.103 | NAD+ | - |
Escherichia coli K-12 |