EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.101 | phosphatidylcholine | micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations | Cavia porcellus | |
1.1.1.101 | phosphatidylglycerol | micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations | Cavia porcellus | |
1.1.1.101 | Triton X-100 | destabilizes during solubilization | Cavia porcellus |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.101 | DTT stabilizes during storage | Cavia porcellus |
1.1.1.101 | freeze-thawing leads to rapid loss of activity | Cavia porcellus |
1.1.1.101 | high ionic strength facilitates solubilization with neutral or anionic detergents | Cavia porcellus |
1.1.1.101 | NADPH stabilizes during storage and solubilization | Cavia porcellus |
1.1.1.101 | Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present | Cavia porcellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.101 | additional information | stable to N-ethylmaleimide | Cavia porcellus | |
1.1.1.101 | N-ethylmaleimide | not inhibitory | Cavia porcellus | |
1.1.1.101 | NADP+ | - |
Cavia porcellus | |
1.1.1.101 | palmitoyl-CoA | competitive to NADPH, not to alkyldihydroxyacetone phosphate | Cavia porcellus | |
1.1.1.101 | Palmitoyldihydroxyacetone phosphate | competitive to hexadecyldihydroxyacetone phosphate | Cavia porcellus | |
1.1.1.101 | PCMB | - |
Cavia porcellus | |
1.1.1.101 | phosphatidylcholine | at high concentrations | Cavia porcellus | |
1.1.1.101 | phosphatidylethanolamine | - |
Cavia porcellus | |
1.1.1.101 | phosphatidylglycerol | at high concentrations | Cavia porcellus | |
1.1.1.101 | phosphatidylinositol | - |
Cavia porcellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.101 | 0.0154 | - |
Palmitoyldihydroxyacetone phosphate | - |
Cavia porcellus | |
1.1.1.101 | 0.02 | - |
NADPH | - |
Cavia porcellus | |
1.1.1.101 | 0.021 | - |
Hexadecyldihydroxyacetone phosphate | - |
Cavia porcellus | |
1.1.1.101 | 1.7 | - |
NADH | - |
Cavia porcellus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.101 | 60000 | - |
1 * 60000, SDS-PAGE | Cavia porcellus |
1.1.1.101 | 70000 | 75000 | gel filtration | Cavia porcellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | Cavia porcellus | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | Cavia porcellus | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.1.1.101 | Glycerol | stabilizes | Cavia porcellus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.101 | Cavia porcellus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.101 | affinity chromatography on NADPH-agarose gel matrix | Cavia porcellus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.101 | liver | - |
Cavia porcellus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.101 | 62.5 | - |
- |
Cavia porcellus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.101 | 4°C, stable in the presence of DTT and NADPH, 1 month | Cavia porcellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
1.1.1.101 | hexadecyl dihydroxyacetone phosphate + NADH + H+ | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NAD+ | - |
r | |
1.1.1.101 | hexadecyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
1.1.1.101 | palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Cavia porcellus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.101 | monomer | 1 * 60000, SDS-PAGE | Cavia porcellus |
1.1.1.101 | More | 27% hydrophobic amino acids by amino acid analysis | Cavia porcellus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.101 | 100 | - |
NADPH | - |
Cavia porcellus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.101 | NADH | can substitute for NADPH | Cavia porcellus | |
1.1.1.101 | NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Cavia porcellus |