BRENDA - Enzyme Database

Purification and properties of acyl/alkyl dihydroxyacetone-phosphate reductase from guinea pig liver peroxisomes

Datta, S.C.; Ghosh, M.K.; Hajra, A.K.; J. Biol. Chem. 265, 8268-8274 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.101
phosphatidylcholine
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
1.1.1.101
phosphatidylglycerol
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
1.1.1.101
Triton X-100
destabilizes during solubilization
Cavia porcellus
General Stability
EC Number
General Stability
Organism
1.1.1.101
DTT stabilizes during storage
Cavia porcellus
1.1.1.101
freeze-thawing leads to rapid loss of activity
Cavia porcellus
1.1.1.101
high ionic strength facilitates solubilization with neutral or anionic detergents
Cavia porcellus
1.1.1.101
NADPH stabilizes during storage and solubilization
Cavia porcellus
1.1.1.101
Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present
Cavia porcellus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.101
additional information
stable to N-ethylmaleimide
Cavia porcellus
1.1.1.101
N-ethylmaleimide
not inhibitory
Cavia porcellus
1.1.1.101
NADP+
-
Cavia porcellus
1.1.1.101
palmitoyl-CoA
competitive to NADPH, not to alkyldihydroxyacetone phosphate
Cavia porcellus
1.1.1.101
Palmitoyldihydroxyacetone phosphate
competitive to hexadecyldihydroxyacetone phosphate
Cavia porcellus
1.1.1.101
PCMB
-
Cavia porcellus
1.1.1.101
phosphatidylcholine
at high concentrations
Cavia porcellus
1.1.1.101
phosphatidylethanolamine
-
Cavia porcellus
1.1.1.101
phosphatidylglycerol
at high concentrations
Cavia porcellus
1.1.1.101
phosphatidylinositol
-
Cavia porcellus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.0154
-
Palmitoyldihydroxyacetone phosphate
-
Cavia porcellus
1.1.1.101
0.02
-
NADPH
-
Cavia porcellus
1.1.1.101
0.021
-
Hexadecyldihydroxyacetone phosphate
-
Cavia porcellus
1.1.1.101
1.7
-
NADH
-
Cavia porcellus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.101
60000
-
1 * 60000, SDS-PAGE
Cavia porcellus
1.1.1.101
70000
75000
gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
1.1.1.101
Glycerol
stabilizes
Cavia porcellus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.101
Cavia porcellus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.101
affinity chromatography on NADPH-agarose gel matrix
Cavia porcellus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.101
62.5
-
-
Cavia porcellus
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.101
4°C, stable in the presence of DTT and NADPH, 1 month
Cavia porcellus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285690
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.101
monomer
1 * 60000, SDS-PAGE
Cavia porcellus
1.1.1.101
More
27% hydrophobic amino acids by amino acid analysis
Cavia porcellus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.101
100
-
NADPH
-
Cavia porcellus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADH
can substitute for NADPH
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Cavia porcellus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.101
phosphatidylcholine
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
1.1.1.101
phosphatidylglycerol
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
1.1.1.101
Triton X-100
destabilizes during solubilization
Cavia porcellus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADH
can substitute for NADPH
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Cavia porcellus
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.101
DTT stabilizes during storage
Cavia porcellus
1.1.1.101
freeze-thawing leads to rapid loss of activity
Cavia porcellus
1.1.1.101
high ionic strength facilitates solubilization with neutral or anionic detergents
Cavia porcellus
1.1.1.101
NADPH stabilizes during storage and solubilization
Cavia porcellus
1.1.1.101
Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present
Cavia porcellus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.101
additional information
stable to N-ethylmaleimide
Cavia porcellus
1.1.1.101
N-ethylmaleimide
not inhibitory
Cavia porcellus
1.1.1.101
NADP+
-
Cavia porcellus
1.1.1.101
palmitoyl-CoA
competitive to NADPH, not to alkyldihydroxyacetone phosphate
Cavia porcellus
1.1.1.101
Palmitoyldihydroxyacetone phosphate
competitive to hexadecyldihydroxyacetone phosphate
Cavia porcellus
1.1.1.101
PCMB
-
Cavia porcellus
1.1.1.101
phosphatidylcholine
at high concentrations
Cavia porcellus
1.1.1.101
phosphatidylethanolamine
-
Cavia porcellus
1.1.1.101
phosphatidylglycerol
at high concentrations
Cavia porcellus
1.1.1.101
phosphatidylinositol
-
Cavia porcellus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.0154
-
Palmitoyldihydroxyacetone phosphate
-
Cavia porcellus
1.1.1.101
0.02
-
NADPH
-
Cavia porcellus
1.1.1.101
0.021
-
Hexadecyldihydroxyacetone phosphate
-
Cavia porcellus
1.1.1.101
1.7
-
NADH
-
Cavia porcellus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.101
60000
-
1 * 60000, SDS-PAGE
Cavia porcellus
1.1.1.101
70000
75000
gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
1.1.1.101
Glycerol
stabilizes
Cavia porcellus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.101
affinity chromatography on NADPH-agarose gel matrix
Cavia porcellus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.101
62.5
-
-
Cavia porcellus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.101
4°C, stable in the presence of DTT and NADPH, 1 month
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285690
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.101
monomer
1 * 60000, SDS-PAGE
Cavia porcellus
1.1.1.101
More
27% hydrophobic amino acids by amino acid analysis
Cavia porcellus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.101
100
-
NADPH
-
Cavia porcellus