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Literature summary extracted from

  • Datta, S.C.; Ghosh, M.K.; Hajra, A.K.
    Purification and properties of acyl/alkyl dihydroxyacetone-phosphate reductase from guinea pig liver peroxisomes (1990), J. Biol. Chem., 265, 8268-8274.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.101 phosphatidylcholine micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations Cavia porcellus
1.1.1.101 phosphatidylglycerol micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations Cavia porcellus
1.1.1.101 Triton X-100 destabilizes during solubilization Cavia porcellus

General Stability

EC Number General Stability Organism
1.1.1.101 DTT stabilizes during storage Cavia porcellus
1.1.1.101 freeze-thawing leads to rapid loss of activity Cavia porcellus
1.1.1.101 high ionic strength facilitates solubilization with neutral or anionic detergents Cavia porcellus
1.1.1.101 NADPH stabilizes during storage and solubilization Cavia porcellus
1.1.1.101 Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.101 additional information stable to N-ethylmaleimide Cavia porcellus
1.1.1.101 N-ethylmaleimide not inhibitory Cavia porcellus
1.1.1.101 NADP+
-
Cavia porcellus
1.1.1.101 palmitoyl-CoA competitive to NADPH, not to alkyldihydroxyacetone phosphate Cavia porcellus
1.1.1.101 Palmitoyldihydroxyacetone phosphate competitive to hexadecyldihydroxyacetone phosphate Cavia porcellus
1.1.1.101 PCMB
-
Cavia porcellus
1.1.1.101 phosphatidylcholine at high concentrations Cavia porcellus
1.1.1.101 phosphatidylethanolamine
-
Cavia porcellus
1.1.1.101 phosphatidylglycerol at high concentrations Cavia porcellus
1.1.1.101 phosphatidylinositol
-
Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.101 0.0154
-
Palmitoyldihydroxyacetone phosphate
-
Cavia porcellus
1.1.1.101 0.02
-
NADPH
-
Cavia porcellus
1.1.1.101 0.021
-
Hexadecyldihydroxyacetone phosphate
-
Cavia porcellus
1.1.1.101 1.7
-
NADH
-
Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.101 60000
-
1 * 60000, SDS-PAGE Cavia porcellus
1.1.1.101 70000 75000 gel filtration Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.101 acyldihydroxyacetone phosphate + NADPH Cavia porcellus enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
1.1.1.101 alkyldihydroxyacetone phosphate + NADPH + H+ Cavia porcellus enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.101 Glycerol stabilizes Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.101 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.101 affinity chromatography on NADPH-agarose gel matrix Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.101 liver
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.101 62.5
-
-
Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
1.1.1.101 4°C, stable in the presence of DTT and NADPH, 1 month Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.101 acyldihydroxyacetone phosphate + NADPH
-
Cavia porcellus 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
1.1.1.101 acyldihydroxyacetone phosphate + NADPH enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis Cavia porcellus 1-acyl-sn-glycerol 3-phosphate + NADP+
-
?
1.1.1.101 alkyldihydroxyacetone phosphate + NADPH + H+
-
Cavia porcellus 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?
1.1.1.101 alkyldihydroxyacetone phosphate + NADPH + H+ enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis Cavia porcellus 1-alkyl-sn-glycerol 3-phosphate + NADP+
-
?
1.1.1.101 hexadecyl dihydroxyacetone phosphate + NADH + H+
-
Cavia porcellus 1-hexadecyl-sn-glycerol 3-phosphate + NAD+
-
r
1.1.1.101 hexadecyldihydroxyacetone phosphate + NADPH
-
Cavia porcellus 1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
?
1.1.1.101 palmitoylglycerone phosphate + NADPH i.e. palmitoyldihydroxyacetone phosphate Cavia porcellus 1-palmitoylglycerol 3-phosphate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.101 monomer 1 * 60000, SDS-PAGE Cavia porcellus
1.1.1.101 More 27% hydrophobic amino acids by amino acid analysis Cavia porcellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.101 100
-
NADPH
-
Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.101 NADH can substitute for NADPH Cavia porcellus
1.1.1.101 NADPH hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates Cavia porcellus