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Literature summary extracted from

  • Caughey, I.; Kekwick, R.G.O.
    The characteristics of some components of the fatty acid synthetase system in the plastids from the mesocarp of avocado (Persea americana) fruit (1982), Eur. J. Biochem., 123, 553-561.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.39 dithiothreitol stabilizes Persea americana
2.3.1.39 EDTA stabilizes Persea americana
2.3.1.39 glycerol, 15% v/v, stabilizes Persea americana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 iodoacetamide
-
 Persea americana
1.3.1.9 N-ethylmaleimide
-
 Persea americana
1.3.1.9 Phenylglyoxal preincubation with NADH prevents whereas NADPH increases the inhibition Persea americana
2.3.1.39 additional information inhibition of the enzyme by thiol-alkylating reagents Persea americana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 additional information
-
 additional information
-
 Persea americana
1.1.1.212 0.00273
-
 NADH
-
 Persea americana
1.1.1.212 0.00965
-
 acetoacetyl-[acyl-carrier protein]
-
 Persea americana
1.1.1.212 0.155
-
 acetoacetyl-CoA
-
 Persea americana
1.1.1.212 6.2
-
 acetoacetyl N-acetyl-cysteamide
-
 Persea americana
1.3.1.9 0.0014 0.0017 crotonyl-[acyl-carrier protein]
-
 Persea americana
1.3.1.9 1.61
-
 Crotonyl-N-acetyl-cysteamine
-
 Persea americana
2.3.1.39 0.00134
-
 pantetheine
-
 Persea americana
2.3.1.39 0.00326
-
 malonyl-CoA
-
 Persea americana
2.3.1.39 0.042
-
 acyl-carrier protein
-
 Persea americana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.9 plastid
-
 Persea americana 9536
-
2.3.1.39 plastid from the mesocarp Persea americana 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 40000 46000 gel filtration, sucrose density gradient centrifugation Persea americana
1.1.1.212 168000
-
 sucrose density gradient centrifugation Persea americana
1.3.1.9 62400
-
 gel filtration Persea americana
2.3.1.39 40500
-
 gel filtration, elution from Sephadex G-100 Persea americana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.212 acetoacetyl-[acyl-carrier protein] + NADH Persea americana component of fatty acid synthetase system 3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein Persea americana the enzyme is a component of the fatty acid synthetase system CoA + malonyl-acyl-carrier protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Persea americana
-
-
-
1.1.1.212 Persea americana
-
 avocado
-
1.3.1.9 Persea americana
-
 avocado
-
2.3.1.39 Persea americana
-
 avocado
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100
-
 Persea americana
1.1.1.212
-
 Persea americana
1.3.1.9
-
 Persea americana
2.3.1.39 ammonium sulfate fractionation and column chromatography on DEAE-cellulose Persea americana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.100 mesocarp
-
 Persea americana
-
1.1.1.212 fruit
-
 Persea americana
-
1.3.1.9 fruit
-
 Persea americana
-
2.3.1.39 mesocarp mesocarp Persea americana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.212 0.0098
-
 12000 x g precipitate of homogenate Persea americana
1.3.1.9 0.068
-
-
 Persea americana
2.3.1.39 9.59
-
-
 Persea americana

Storage Stability

EC Number Storage Stability Organism
1.1.1.212 -20°C, 50 mM phosphate buffer, pH 7.0, 10% glycerol, stable for two weeks Persea americana
1.3.1.9 20°C, 0.1 M phosphate buffer, pH 7.0, unstable Persea americana
2.3.1.39 -20°C, potassium-phosphate buffer, pH: 7.0, 1 mM dithiothreitol, 0.5 mM EDTA, 15% v/v glycerol Persea americana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Persea americana (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.212 acetoacetyl-CoA + NADH
-
 Persea americana 3-hydroxybutanoyl-CoA + NAD+
-
 ?
1.1.1.212 acetoacetyl-N-acetylcysteamine + NADH very poor substrate Persea americana 3-hydroxybutanoyl-N-acetylcysteamine + NAD+
-
 ?
1.1.1.212 acetoacetyl-[acyl-carrier protein] + NADH
-
 Persea americana 3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.1.1.212 acetoacetyl-[acyl-carrier protein] + NADH component of fatty acid synthetase system Persea americana 3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 crotonyl-N-acetyl-cysteamine + NADH
-
 Persea americana butyryl-N-acetyl-cysteamine + NAD+
-
 ?
1.3.1.9 crotonyl-[acyl-carrier protein] + NADH + H+
-
 Persea americana butyryl-[acyl-carrier protein] + NAD+
-
 r
1.3.1.9 trans-2-decenoyl-[acyl-carrier protein] + NADH
-
 Persea americana decanoyl-[acyl-carrier protein] + NAD+
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Persea americana CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein the enzyme is a component of the fatty acid synthetase system Persea americana CoA + malonyl-acyl-carrier protein
-
 ?
2.3.1.39 malonyl-CoA + pantetheine
-
 Persea americana malonyl-pantetheine + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 30
-
 assay at Persea americana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.100 6.5
-
-
 Persea americana
1.1.1.212 7
-
-
 Persea americana
1.3.1.9 6 7.5
-
 Persea americana
2.3.1.39 7
-
-
 Persea americana
2.3.1.39 8.3
-
-
 Persea americana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.100 5.5 8.5 about 50% of activity maximum at pH 5.5 and 8.5 Persea americana
1.1.1.212 6 8
-
 Persea americana
2.3.1.39 6.2 9.8 about half-maximal activity at pH 6.2, about 65% of maximal activity at pH 9.8 Persea americana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.9 6 7.5
-
 Persea americana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 additional information NADH is used by NADH-dependent 3-oxoacyl [ACP]-reductase, EC 1.1.1.121 Persea americana
1.1.1.100 NADPH
-
 Persea americana
1.1.1.212 NADH
-
 Persea americana
1.3.1.9 NADH absolute specificity Persea americana