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Literature summary extracted from

  • Sheldon, P.S.; Kekwick, R.G.O.; Sidebottom, C.; Smith, C.G.; Slabas, A.R.
    3-Oxoacyl-(acyl-carrier protein) reductase from avocado (Persea americana) fruit mesocarp (1990), Biochem. J., 271, 713-720.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.100 inactivation by dilution can be partly prevented by inclusion of NADPH Persea americana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.100 Phenylglyoxal with NADPH 45% remaining activity, without total loss of activity Persea americana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.0079
-
 acetoacetyl-[acyl-carrier protein]
-
 Persea americana
1.1.1.100 0.0093
-
 NADPH
-
 Persea americana
1.1.1.100 0.47
-
 acetoacetyl-CoA
-
 Persea americana
1.1.1.100 48
-
 acetoacetyl-N-acetylcysteamine
-
 Persea americana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 28000
-
 4 * 28000, SDS-PAGE Persea americana
1.1.1.100 130000
-
 gel filtration Persea americana

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Persea americana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100
-
 Persea americana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.100 mesocarp
-
 Persea americana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.100 280
-
 acetoacetyl-N-acetylcysteamine Persea americana
1.1.1.100 450
-
 acetoacetyl-CoA Persea americana
1.1.1.100 530
-
 acetoacteyl-ACP Persea americana
1.1.1.100 9401
-
 purified enzyme, protein determination by amino acid analysis Persea americana

Storage Stability

EC Number Storage Stability Organism
1.1.1.100 27°C, 2 M NaCl, 24 h stable Persea americana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Persea americana (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH + H+
-
 Persea americana D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.100 acetoacetyl-N-acetylcysteamine + NADPH + H+
-
 Persea americana D-3-hydroxybutyryl-N-acetylcysteamine + NADP+
-
 r
1.1.1.100 acetoacetyl-N-cysteamine + NADPH
-
 Persea americana D-3-hydroxybutyryl-N-cysteamine + NADP+
-
 ?
1.1.1.100 acetoacetyl-[acyl-carrier protein] + NADPH
-
 Persea americana D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 tetramer 4 * 28000, SDS-PAGE Persea americana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Persea americana