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Literature summary extracted from

  • Shimakata, T.; Stumpf, P.K.
    The procaryotic nature of the fatty acid synthetase of developing Carthamus tinctorius L. (Safflower) seeds (1982), Arch. Biochem. Biophys., 217, 144-154.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 cerulenin
-
 Carthamus tinctorius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.009
-
 acetoacetyl-[acyl-carrier protein]
-
 Carthamus tinctorius
1.1.1.100 0.016
-
 NADPH
-
 Carthamus tinctorius
1.3.1.9 0.0014 0.0017 crotonyl-[acyl-carrier protein] reductase I Carthamus tinctorius
1.3.1.9 0.003
-
 NADPH reductase II Carthamus tinctorius
1.3.1.9 0.0059
-
 NADH reductase I Carthamus tinctorius
1.3.1.9 0.007
-
 trans-2-decenoyl-[acyl-carrier protein]
-
 Carthamus tinctorius
1.3.1.9 0.007
-
 2-decenoyl-[acyl-carrier protein] reductase I Carthamus tinctorius
1.3.1.9 0.014
-
 2-decenoyl-[acyl-carrier protein] reductase I Carthamus tinctorius
1.3.1.9 0.667
-
 crotonyl-CoA reductase I Carthamus tinctorius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.9 83000
-
 gel filtration Carthamus tinctorius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH Carthamus tinctorius enzyme in fatty acid synthesis (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.3.1.10 additional information Carthamus tinctorius part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters ?
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] Carthamus tinctorius part of non-associated fatty acid synthase system of plants and prokaryotes CoA + acetyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Carthamus tinctorius
-
 safflower
-
1.3.1.9 Carthamus tinctorius
-
 safflower
-
1.3.1.10 Carthamus tinctorius
-
 safflower, enoyl-ACP reductase II, as there is no information concerning the stereochemistry of hydrogen transfer from NADPH to substrate an appointment to EC 1.3.1.10 or EC 1.3.1.39 is impossible
-
2.3.1.38 Carthamus tinctorius
-
 safflower
-
2.3.1.39 Carthamus tinctorius
-
 safflower
-
2.3.1.41 Carthamus tinctorius
-
 safflower
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 two different forms I and II Carthamus tinctorius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.100 seed
-
 Carthamus tinctorius
-
1.3.1.9 seed
-
 Carthamus tinctorius
-
2.3.1.38 seed
-
 Carthamus tinctorius
-
2.3.1.39 seed
-
 Carthamus tinctorius
-
2.3.1.41 seed germinating Carthamus tinctorius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.100 0.0197
-
-
 Carthamus tinctorius
1.3.1.9 0.00608
-
 reductase II Carthamus tinctorius
1.3.1.9 0.0153
-
 reductase I Carthamus tinctorius
2.3.1.38 0.00023
-
 crude extract Carthamus tinctorius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Carthamus tinctorius (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH enzyme in fatty acid synthesis Carthamus tinctorius (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 acetoacetyl-[acyl-carrier protein] + NADPH
-
 Carthamus tinctorius D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
 ?
1.3.1.9 2-decenoyl-[acyl-carrier protein] + NADH
-
 Carthamus tinctorius decanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 crotonyl-CoA + NADH only reductase I Carthamus tinctorius butyryl-CoA + NAD+
-
 ?
1.3.1.9 crotonyl-CoA + NADPH + H+
-
 Carthamus tinctorius butyryl-CoA + NADP+
-
 ?
1.3.1.9 crotonyl-[acyl-carrier protein] + NADH
-
 Carthamus tinctorius butyryl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 additional information enoyl [acyl-carrier-protein] I uses NADH, whereas enoyl [acyl-carrier-protein] II uses NADPH Carthamus tinctorius ?
-
 ?
1.3.1.9 trans-2-decenoyl-[acyl-carrier protein] + NADH
-
 Carthamus tinctorius decanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.10 additional information part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters Carthamus tinctorius ?
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Carthamus tinctorius CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] part of non-associated fatty acid synthase system of plants and prokaryotes Carthamus tinctorius CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Carthamus tinctorius CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Carthamus tinctorius 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.9 6.5
-
 reductase I Carthamus tinctorius
1.3.1.9 7.1
-
 reductase II Carthamus tinctorius
2.3.1.38 25
-
 assay at Carthamus tinctorius

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Carthamus tinctorius
1.3.1.9 NADH reductase I Carthamus tinctorius
1.3.1.9 NADPH reductase II Carthamus tinctorius