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Literature summary extracted from

  • Shimakata, T.; Stumpf, P.K.
    Purification and characterizations of beta-ketoacyl-[acyl-carrier-protein] reductase, beta-hydroxyacyl-[acylcarrier-protein] dehydrase, and enoyl-[acyl-carrier-protein] reductase from Spinacia oleracea leaves (1982), Arch. Biochem. Biophys., 218, 77-91.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.100 additional information not: N-ethylmaleimide, arsenite Spinacia oleracea
1.1.1.100 p-chloromercuribenzoate
-
 Spinacia oleracea
1.3.1.9 additional information not inhibitory: arsenite Spinacia oleracea
1.3.1.9 N-ethylmaleimide not Spinacia oleracea
1.3.1.9 p-chloromercuribenzoate 87% inhibition at 0.1 mM Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.0037
-
 acetoacetyl-[acyl-carrier protein]
-
 Spinacia oleracea
1.1.1.100 0.025
-
 NADPH
-
 Spinacia oleracea
1.1.1.100 0.25
-
 acetoacetyl-CoA
-
 Spinacia oleracea
1.3.1.9 0.0029
-
 2-hexadecenoyl-[acyl-carrier protein]
-
 Spinacia oleracea
1.3.1.9 0.00552
-
 2-decenoyl-[acyl-carrier protein]
-
 Spinacia oleracea
1.3.1.9 0.0062
-
 2-hexenoyl-[acyl-carrier protein]
-
 Spinacia oleracea
1.3.1.9 0.0097
-
 crotonyl-[acyl-carrier protein]
-
 Spinacia oleracea
1.3.1.9 0.011
-
 NADH
-
 Spinacia oleracea
1.3.1.9 0.0133
-
 2-octenoyl-[acyl-carrier protein]
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 24200
-
 4 * 24200, SDS-PAGE Spinacia oleracea
1.1.1.100 97000
-
 gel filtration Spinacia oleracea
1.3.1.9 32500
-
 monomeric form, SDS-PAGE Spinacia oleracea
1.3.1.9 115000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Escherichia coli
-
-
-
1.1.1.100 Spinacia oleracea
-
-
-
1.3.1.9 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100
-
 Spinacia oleracea
1.3.1.9 homogeneity Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.100 leaf
-
 Spinacia oleracea
-
1.3.1.9 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.100 0.0032
-
-
 Escherichia coli
1.1.1.100 0.005
-
-
 Spinacia oleracea
1.1.1.100 7.95
-
-
 Spinacia oleracea
1.3.1.9 63.69
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.1.1.100 -20°C, stable for 2 months Spinacia oleracea
1.3.1.9 -20°C, 2 months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Escherichia coli (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 r
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Spinacia oleracea (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 r
1.1.1.100 acetoacetyl-CoA + NADPH + H+
-
 Escherichia coli D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH + H+
-
 Spinacia oleracea D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.100 acetoacetyl-[acyl-carrier protein] + NADPH
-
 Escherichia coli D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
 r
1.1.1.100 acetoacetyl-[acyl-carrier protein] + NADPH
-
 Spinacia oleracea D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
 r
1.1.1.100 additional information L-isomer completely inactive Escherichia coli ?
-
 ?
1.1.1.100 additional information L-isomer completely inactive Spinacia oleracea ?
-
 ?
1.3.1.9 2-decenoyl-[acyl-carrier protein] + NADH
-
 Spinacia oleracea decanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 2-dodecenoyl-[acyl-carrier protein] + NADH
-
 Spinacia oleracea dodecanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 2-hexadecenoyl-[acyl-carrier protein] + NADH
-
 Spinacia oleracea hexadecanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 2-hexenoyl-[acyl-carrier protein] + NADH fast reduction Spinacia oleracea hexanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 2-octenoyl-[acyl-carrier protein] + NADH fast reduction Spinacia oleracea octanoyl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 crotonyl-[acyl-carrier protein] + NADH
-
 Spinacia oleracea butyryl-[acyl-carrier protein] + NAD+
-
 ?
1.3.1.9 additional information no reduction of 3-decenoyl-[acyl-carrier-protein] Spinacia oleracea ?
-
 ?
1.3.1.9 additional information substrate specificity: acyl chain length C4-C16 Spinacia oleracea ?
-
 ?
1.3.1.9 trans-2-decenoyl-[acyl-carrier protein] + NADH
-
 Spinacia oleracea decanoyl-[acyl-carrier protein] + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 tetramer 4 * 24200, SDS-PAGE Spinacia oleracea
1.3.1.9 tetramer 4 * 32500, SDS-PAGE Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.9 6.4
-
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.9 6.4
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.100 6 8.7 both reaction directions Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADH NADPH is much more effective than NADH Spinacia oleracea
1.1.1.100 NADP+
-
 Escherichia coli
1.1.1.100 NADP+
-
 Spinacia oleracea
1.1.1.100 NADPH
-
 Escherichia coli
1.1.1.100 NADPH
-
 Spinacia oleracea
1.3.1.9 NADH
-
 Spinacia oleracea