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Literature summary extracted from

  • Hensgens, C.M.H.; Vonck, J.; van Beeumen, J.; Bruggen, E.F.J.; Hansen, T.A.
    Purification and characterization of an oxygen-labile, NAD-dependent alcohol dehydrogenase from Desulfovibrio gigas (1993), J. Bacteriol., 175, 2859-2863.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 2,2'-dipyridyl
-
 Megalodesulfovibrio gigas
1.1.1.1 Co2+
-
 Megalodesulfovibrio gigas
1.1.1.1 EDTA
-
 Megalodesulfovibrio gigas
1.1.1.1 Mn2+
-
 Megalodesulfovibrio gigas
1.1.1.1 NEM
-
 Megalodesulfovibrio gigas
1.1.1.1 Ni2+
-
 Megalodesulfovibrio gigas
1.1.1.1 p-chloromercuribenzene sulfonate
-
 Megalodesulfovibrio gigas
1.1.1.1 PCMB
-
 Megalodesulfovibrio gigas
1.1.1.1 Zn2+
-
 Megalodesulfovibrio gigas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.02
-
 NADH reduction of acetaldehyde Megalodesulfovibrio gigas
1.1.1.1 0.074
-
 NAD+ oxidation of ethanol Megalodesulfovibrio gigas
1.1.1.1 0.15
-
 ethanol
-
 Megalodesulfovibrio gigas
1.1.1.1 0.28
-
 propan-1-ol
-
 Megalodesulfovibrio gigas
1.1.1.1 1.8
-
 acetaldehyde
-
 Megalodesulfovibrio gigas
1.1.1.1 50
-
 formaldehyde
-
 Megalodesulfovibrio gigas

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zinc contains 1.2 Zn atom per subunit Megalodesulfovibrio gigas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 43000
-
 10 * 43000, SDS-PAGE Megalodesulfovibrio gigas
1.1.1.1 320000
-
 gel filtration Megalodesulfovibrio gigas

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Megalodesulfovibrio gigas
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.1 pronounced oxygen lability at pH 9, even at pH 7, 2 h under air, 80% loss of activity Megalodesulfovibrio gigas

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 31.8
-
-
 Megalodesulfovibrio gigas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1,3-propanediol + NAD+ 7% of the activity with ethanol Megalodesulfovibrio gigas ? + NADH
-
 ?
1.1.1.1 acetaldehyde + NADH + H+ the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol Megalodesulfovibrio gigas ethanol + NAD+
-
 r
1.1.1.1 butyraldehyde + NADH
-
 Megalodesulfovibrio gigas n-butanol + NAD+
-
 r
1.1.1.1 ethanol + NAD+ the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol Megalodesulfovibrio gigas acetaldehyde + NADH
-
 r
1.1.1.1 formaldehyde + NADH + H+
-
 Megalodesulfovibrio gigas methanol + NAD+
-
 ?
1.1.1.1 propan-1,2-diol + NAD+
-
 Megalodesulfovibrio gigas ? + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+ 3% of the activity with ethanol Megalodesulfovibrio gigas acetone + NADH
-
 ?
1.1.1.1 propanol + NAD+
-
 Megalodesulfovibrio gigas propionaldehyde + NADH + H+
-
 ?
1.1.1.1 propionaldehyde + NADH
-
 Megalodesulfovibrio gigas propanol + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 decamer 10 * 43000, SDS-PAGE Megalodesulfovibrio gigas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 40
-
-
 Megalodesulfovibrio gigas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 7.5
-
 reduction of acetaldehyde Megalodesulfovibrio gigas
1.1.1.1 9.5
-
 oxidation of ethanol Megalodesulfovibrio gigas

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Megalodesulfovibrio gigas
1.1.1.1 NADH
-
 Megalodesulfovibrio gigas
1.1.1.1 NADP+ at 1.5% of the activity with NAD+ Megalodesulfovibrio gigas
1.1.1.1 NADPH at 1.6% of the activity with NADH Megalodesulfovibrio gigas