Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yabe, M.; Shitara, K.; Kawashima, J.; Shinoyama, H.; Ando, A.; Fujii, T.
    Purification and properties of an alcohol dehydrogenase isozyme from a methanol-using yeast, Candida sp. N-16 (1992), Biosci. Biotechnol. Biochem., 56, 338-339.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 EDTA enhances activity Candida sp. (in: Saccharomycetales)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 Zn2+ 0.1 mM, 20% decrease of activity Candida sp. (in: Saccharomycetales)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.195
-
 NAD+
-
 Candida sp. (in: Saccharomycetales)
1.1.1.1 0.487
-
 NAD+
-
 Candida sp. (in: Saccharomycetales)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Fe2+ enhances activity Candida sp. (in: Saccharomycetales)
1.1.1.1 Mg2+ enhances activity Candida sp. (in: Saccharomycetales)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 45000
-
 4 * 45000, SDS-PAGE Candida sp. (in: Saccharomycetales)
1.1.1.1 180000
-
 gel filtration Candida sp. (in: Saccharomycetales)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.1 additional information Candida sp. (in: Saccharomycetales) one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII ?
-
 ?
1.1.1.1 additional information Candida sp. (in: Saccharomycetales) N-16 one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Candida sp. (in: Saccharomycetales)
-
 N-16
-
1.1.1.1 Candida sp. (in: Saccharomycetales) N-16
-
 N-16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 ADH-MII Candida sp. (in: Saccharomycetales)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 49.3
-
-
 Candida sp. (in: Saccharomycetales)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 acetaldehyde + NADH + H+
-
 Candida sp. (in: Saccharomycetales) ethanol + NAD+
-
 ?
1.1.1.1 acetaldehyde + NADH + H+
-
 Candida sp. (in: Saccharomycetales) N-16 ethanol + NAD+
-
 ?
1.1.1.1 ethanol + NAD+ the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol Candida sp. (in: Saccharomycetales) acetaldehyde + NADH
-
 r
1.1.1.1 ethanol + NAD+ the reduction of acetaldehyde of ADH-MII is about 7times higher than that of the oxidation of ethanol Candida sp. (in: Saccharomycetales) N-16 acetaldehyde + NADH
-
 r
1.1.1.1 methanol + NAD+ reaction is extremly weak Candida sp. (in: Saccharomycetales) formaldehyde + NADH + H+
-
 ?
1.1.1.1 methanol + NAD+ reaction is extremly weak Candida sp. (in: Saccharomycetales) N-16 formaldehyde + NADH + H+
-
 ?
1.1.1.1 additional information one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII Candida sp. (in: Saccharomycetales) ?
-
 ?
1.1.1.1 additional information one constitutive enzyme, ADH-MI and one inducible enzyme, ADH-MII Candida sp. (in: Saccharomycetales) N-16 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 tetramer 4 * 45000, SDS-PAGE Candida sp. (in: Saccharomycetales)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 70
-
 above Candida sp. (in: Saccharomycetales)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 7
-
-
 Candida sp. (in: Saccharomycetales)

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Candida sp. (in: Saccharomycetales)
1.1.1.1 NADH
-
 Candida sp. (in: Saccharomycetales)