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Literature summary extracted from

  • Lai, Y.K.; Chandlee, J.M.; Scandalios, J.G.
    Purification and characterization of three non-allelic alcohol dehydrogenase isoenzymes in maize (1982), Biochim. Biophys. Acta, 706, 9-18.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 1,10-phenanthroline
-
Zea mays
1.1.1.1 Cu2+ no effect Zea mays
1.1.1.1 Hg2+ no effect Zea mays
1.1.1.1 iodoacetamide
-
Zea mays
1.1.1.1 NEM
-
Zea mays
1.1.1.1 p-hydroxymercuribenzoate
-
Zea mays
1.1.1.1 PCMB
-
Zea mays
1.1.1.1 PMSF
-
Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 additional information
-
additional information
-
Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zinc required for activity, tightly bound within the enzyme Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 35000
-
2 * 35000, SDS-PAGE Zea mays
1.1.1.1 70000
-
gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Zea mays
-
3 non-allelic alcohol dehydrogenase isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 scutellum
-
Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 additional information
-
-
Zea mays

Storage Stability

EC Number Storage Stability Organism
1.1.1.1 -20°C, isoenzyme 2 is stable in 50% ethylene glycol for at least 3 months, isoenzyme 1 and 3 maintain less than 50% of their original activity after 2 weeks Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 2-butene-1-ol + NAD+
-
Zea mays ? + NADH
-
?
1.1.1.1 2-methylpropan-1-ol + NAD+ weak activity Zea mays 2-methyl-propan-1-one + NADH
-
?
1.1.1.1 3-butene-1-ol + NAD+
-
Zea mays ? + NADH
-
?
1.1.1.1 3-phenyl-2-propen-1-ol + NAD+
-
Zea mays ? + NADH
-
?
1.1.1.1 allyl alcohol + NAD+
-
Zea mays prop-2-en-1-al + NADH
-
?
1.1.1.1 benzyl alcohol + NAD+ no activity Zea mays benzaldehyde + NADH
-
?
1.1.1.1 butan-2-ol + NAD+ weak activity Zea mays butan-2-one + NADH
-
?
1.1.1.1 butanol + NAD+
-
Zea mays butyraldehyde + NADH
-
?
1.1.1.1 cyclohexanol + NAD+ no activity Zea mays cyclohexanone + NADH
-
?
1.1.1.1 ethanol + NAD+
-
Zea mays acetaldehyde + NADH
-
?
1.1.1.1 hexan-1-ol + NAD+
-
Zea mays n-hexanal + NADH
-
?
1.1.1.1 methanol + NAD+ no activity Zea mays formaldehyde + NADH + H+
-
?
1.1.1.1 octan-1-ol + NAD+
-
Zea mays n-octanal + NADH
-
?
1.1.1.1 pentanol + NAD+
-
Zea mays n-pentanal + NADH
-
?
1.1.1.1 propanol + NAD+
-
Zea mays propionaldehyde + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 35000, SDS-PAGE Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 55
-
60 min, 73% loss of activity of enzyme 1, 10% loss of activity of enzyme 2, 21% loss of activity of enzyme 3 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 10
-
-
Zea mays

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.1 6 9 isoenzyme 2 and isoenzyme 3 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
Zea mays
1.1.1.1 NADH
-
Zea mays