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Literature summary extracted from

  • Ditlow, C.C.; Holmquist, B.; Morelock, M.M.; Vallee, B.L.
    Physical and enzymatic properties of a class II alcohol dehydrogenase isozyme of human liver: pi-ADH (1984), Biochemistry, 23, 6363-6368.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 1,10-phenanthroline
-
 Homo sapiens
1.1.1.1 2,2'-bipyridine
-
 Homo sapiens
1.1.1.1 4-Methylpyrazole poor inhibitor, class II isoenzyme Homo sapiens
1.1.1.1 EDTA
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.007
-
 benzyl alcohol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 0.007
-
 Octanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 0.032
-
 3-Phenyl-1-propanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 0.034
-
 Vanillyl alcohol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 0.06
-
 16-hydroxyhexadecanoate 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 0.09
-
 Pentanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 0.2
-
 tryptophol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 0.23
-
 12-hydroxydodecanoate 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 120
-
 ethanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 210
-
 Cyclohexanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 290
-
 ethylene glycol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 310
-
 2-deoxy-D-ribose 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens
1.1.1.1 560
-
 2-propanol 0.1 M glycine-NaOH buffer, pH 10.0, 25°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 42000
-
 x * 42000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Homo sapiens
-
 class II isoenzyme: pi-ADH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 class II isoenzyme: pi-ADH Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 1.47
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.1 4°C, pH 7.5, stable for 2-3 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 12-hydroxydodecanoate + NAD+
-
 Homo sapiens 12-oxododecanoic acid + NADH
-
 ?
1.1.1.1 16-hydroxyhexadecanoate + NAD+
-
 Homo sapiens 16-oxohexadecanoic acid + NADH
-
 ?
1.1.1.1 2-deoxy-D-ribose + NAD+
-
 Homo sapiens ? + NADH
-
 ?
1.1.1.1 3-phenyl-1-propanol + NAD+
-
 Homo sapiens 3-phenyl-1-propanone + NADH
-
 ?
1.1.1.1 benzyl alcohol + NAD+
-
 Homo sapiens benzaldehyde + NADH
-
 ?
1.1.1.1 cyclohexanol + NAD+
-
 Homo sapiens cyclohexanone + NADH
-
 ?
1.1.1.1 ethanol + NAD+
-
 Homo sapiens acetaldehyde + NADH
-
 ?
1.1.1.1 ethylene glycol + NAD+
-
 Homo sapiens ? + NADH
-
 ?
1.1.1.1 methanol + NAD+ activity detected with class II isoenzyme pi-ADH Homo sapiens formaldehyde + NADH + H+
-
 ?
1.1.1.1 octan-1-ol + NAD+
-
 Homo sapiens n-octanal + NADH
-
 ?
1.1.1.1 pentanal + NAD+
-
 Homo sapiens pentanone + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+
-
 Homo sapiens acetone + NADH
-
 ?
1.1.1.1 tryptophol + NAD+
-
 Homo sapiens ? + NADH
-
 ?
1.1.1.1 vanillyl alcohol + NAD+
-
 Homo sapiens vanillin + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 ? x * 42000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.583
-
 Cyclohexanol
-
 Homo sapiens
1.1.1.1 0.75
-
 2-propanol
-
 Homo sapiens
1.1.1.1 0.75
-
 ethylene glycol
-
 Homo sapiens
1.1.1.1 1.83
-
 tryptophol
-
 Homo sapiens
1.1.1.1 2.83
-
 2-deoxy-D-ribose
-
 Homo sapiens
1.1.1.1 4
-
 12-hydroxydodecanoate
-
 Homo sapiens
1.1.1.1 6.17
-
 16-hydroxyhexadecanoate
-
 Homo sapiens
1.1.1.1 7.5
-
 3-Phenyl-1-propanol
-
 Homo sapiens
1.1.1.1 7.83
-
 ethanol
-
 Homo sapiens
1.1.1.1 8
-
 Pentanol
-
 Homo sapiens
1.1.1.1 8.33
-
 Octanol
-
 Homo sapiens
1.1.1.1 8.67
-
 Vanillyl alcohol
-
 Homo sapiens
1.1.1.1 9.17
-
 benzyl alcohol
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 10.4
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 8 12 about 30% of maximal activity at pH 8.0 and at pH 12.0 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Homo sapiens
1.1.1.1 NADH
-
 Homo sapiens